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  • Recombinant Mouse Anti-SMURF1 Antibody (clone 1A9H4)

Recombinant Mouse Anti-SMURF1 Antibody (clone 1A9H4) (CAT#: VS4-CJ122)

This mouse anti-SMURF1 antibody (clone 1A9H4) is a product that recognizes human SMURF1. The immunogen of this antibody is recombinant human E3 ubiquitin-protein ligase SMURF1 protein (198aa-374aa). This product was purified by protein G purified and it has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).


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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1924_A2_3_selected.jpg
Figure 2 IHC staining of human esophagus
Figure 2 IHC staining of human esophagus
(Immunohistochemical staining of human esophagus shows membrane positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_B_6_1_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1841_E2_1_blue_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1899_D1_5_cr5ba0db0f311d8_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_B_7_5.jpg
Figure 6 Colon
Figure 6 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_8_3.jpg
Figure 7 Liver
Figure 7 Liver
(Cholangiocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_9_4.jpg
Figure 8 Kidney
Figure 8 Kidney
(Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_9_5.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (A-431, BJ, EFO-21, HDLM-2, PC-3, U-2 OS, U-251 MG)
Figure 9 RNA cell line category: Cell line enhanced (A-431, BJ, EFO-21, HDLM-2, PC-3, U-2 OS, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198742-SMURF1

Specifications

  • Immunogen
  • Recombinant Human E3 ubiquitin-protein ligase SMURF1 protein (198aa-374aa)
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2a
  • Specificity
  • Human SMURF1
  • Species Reactivity
  • Human
  • Clone
  • 1A9H4
  • Applications
  • ELISA, WB, IHC, IF, FC
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Protein G purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • 50% Glycerol, PBS, pH 7.3.
  • Preservative
  • 0.1% Sodium Azide
  • Storage
  • Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

Applications

  • Application Notes
  • This antibody has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).

Target

  • Alternative Names
  • SMAD specific E3 ubiquitin protein ligase 1
  • Long Name
  • SMAD specific E3 ubiquitin protein ligase 1
  • Cellular Localization
  • Cell membrane, Cytoplasm, Membrane
  • Post Translation Modifications
  • Auto-ubiquitinated in presence of NDFIP1 (PubMed:23087404).
    Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
  • Function
  • E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404).
    Acts as an antagonist of TGF-beta signaling by ubiquitinating TGFBR1 and targeting it for degradation (PubMed:21791611).
    Plays a role in dendrite formation by melanocytes (PubMed:23999003).

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "SMURF1"

Recombinant Antibody

CAT Product Name Application Type
DrMAB-553 Recombinant Human Anti-Human SMURF1 Antibody, clone D560 IP, IF, ELISA IgG
MOB-1894CT Recombinant Mouse anti-Human SMURF1 Monoclonal antibody (EML1749) ICC/IF, IHC-P, WB

Fab Fragment Antibody

CAT Product Name Application Type
DrMAB-554 Recombinant Human Anti-Human SMURF1 Antibody Fab Fragment, clone D562 IP, IF, ELISA, IP-MS Fab

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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