Recombinant Mouse Anti-SYP Antibody (clone 3E3-1E6-3E10) (CAT#: VS3-FY1420)

• Immunogen
• Synthetic peptides derived from human synaptophysin.

• Host Species
• Mouse

• Type
• Mouse IgG2a

• Specificity
• Human SYP

• Species Reactivity
• Human

• Clone
• 3E3-1E6-3E10

• Applications
• Immunohistochemistry-Paraffin

• Conjugate
• Unconjugated

• Purification
• Affinity Chromatography

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS, pH 7.3, 50% glycerol, 0.5% BSA

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Immunohistochemistry-Paraffin: 1/100-1/500

• Alternative Names
• SYP; Synaptophysin; Major synaptic vesicle protein p38

• Gene ID
• 6855

• UniProt ID
• P08247

• Sequence Similarities
• Belongs to the synaptophysin/synaptobrevin family.

• Cellular Localization
• Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

• Post Translation Modifications
• Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  Phosphorylated by SRC.

• Protein Refseq
• NP_003170.1

• Function
• Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

Secondary Antibody

• 2ND-048CQ
• Goat Anti-Mouse IgG2a Antibody, R-PE

• 2RD-A1464-YJ
• Goat Anti-Mouse IgG2a Secondary antibody

• 2RD-A1564-YJ
• Rabbit Anti-Mouse IgG2a Secondary antibody (Clone RM219)

Isotype Control

• MOB-202CQ
• Mouse IgG2a, kappa Isotype Control Antibody

Reconstitution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS