Recombinant Rabbit Anti-SYP Antibody (clone R06-2A2) (CAT#: VS3-FY1421)

This product is a recombinant rabbit antibody that recognizes SYP. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R06-2A2 is specific for human, rat SYP. The antigen is synthetic peptide of human synaptophysin.


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ICC

Figure 1. Recombinant Rabbit Anti-SYP Antibody (clone R06-2A2) in ICC.

Figure 1. Recombinant Rabbit Anti-SYP Antibody (clone R06-2A2) in ICC.

Immunocytochemical analysis of synaptophysin (green) in 3T3 using synaptophysin antibody and DAPI (blue).


Specifications

  • Immunogen
  • Synthetic peptide of human synaptophysin.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Rat SYP
  • Species Reactivity
  • Human, Rat
  • Clone
  • R06-2A2
  • Applications
  • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 34 kDa; Observed MW: 38 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry-Paraffin: 1/50-1/100
    Immunofluorescence: 1/50-1/200

Target

  • Alternative Names
  • SYP; Synaptophysin; Major synaptic vesicle protein p38
  • Sequence Similarities
  • Belongs to the synaptophysin/synaptobrevin family.
  • Cellular Localization
  • Cytoplasmic vesicle, Membrane, Synapse, Synaptosome
  • Post Translation Modifications
  • Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
    Phosphorylated by SRC.
  • Function
  • Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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