Recombinant monoclonal antibody to CD22. Clone MJ-7 is a human monoclonal antibody that can be potentially used in the treatment of B-cell malignancy.
Figure 1 Western blot analysis of purified MJ-7.
Size of molecular weight markers are indicated (kDa).
Arndt, M. A., Krauss, J., Schwarzenbacher, R., Vu, B. K., Greene, S., & Rybak, S. M. (2003). Generation of a highly stable, internalizing anti‐CD22 single‐chain Fv fragment for targeting non‐Hodgkin's lymphoma. International journal of cancer, 107(5), 822-829.
Figure 2 Immunoreactivity of monomeric MJ-7 (closed triangle) and MLZ-wt (closed square) after incubation in human serum at 37°C over a period of 6 days.
Arndt, M. A., Krauss, J., Schwarzenbacher, R., Vu, B. K., Greene, S., & Rybak, S. M. (2003). Generation of a highly stable, internalizing anti‐CD22 single‐chain Fv fragment for targeting non‐Hodgkin's lymphoma. International journal of cancer, 107(5), 822-829.
Figure 3 Biophysical stability of IMAC-purified scFv variants.
Immunoreactivity with CD22+ Raji cells was assayed by flow cytometry after incubating the constructs at 37°C in human serum for various time periods. The half-life was determined as the time point with 50% of the initial binding activity.
Arndt, M. A., Krauss, J., Schwarzenbacher, R., Vu, B. K., Greene, S., & Rybak, S. M. (2003). Generation of a highly stable, internalizing anti‐CD22 single‐chain Fv fragment for targeting non‐Hodgkin's lymphoma. International journal of cancer, 107(5), 822-829.
Figure 4 Affinity and specificity of wild-type scFv and stability engineered scFv.
(a) Equilibrium-binding curves for MAb LL2 (open circle); MLZ-wt (closed square) and MJ-7 (closed triangle) as determined by flow cytometry. Binding activity to Raji cells at indicated concentrations is shown as median fluorescence intensity (MFI) minus background fluorescence. Measurements were performed in triplicate; standard deviations are shown as bars. Binding affinity constants (Kd) were determined by fitting the cell binding data to the nonlinear regression model according to the Levenberg-Marquard method. (b) Epitope specificity was determined by competition experiments and analyzed by flow cytometry. MLZ-wt (closed square) and MJ-7 (closed triangle) competed with the MAb LL2 for binding to the CD22 antigen on Raji cells. Results are shown as percent binding inhibition of MAb LL2 at a concentration of 100 nM when incubating tumor cells with various concentrations of scFvs.
Arndt, M. A., Krauss, J., Schwarzenbacher, R., Vu, B. K., Greene, S., & Rybak, S. M. (2003). Generation of a highly stable, internalizing anti‐CD22 single‐chain Fv fragment for targeting non‐Hodgkin's lymphoma. International journal of cancer, 107(5), 822-829.
Figure 5 Internalization of antibodies into living tumor cells.
Alexa-488-labeled MAb LL2 (a,b) or scFv MJ-7 (c,d), each at a concentration of 50 μg/ml, were incubated with Raji cells (5 × 10⁵) for 30 min either at 4°C (a,c) or 37°C (b,d) before analysis by confocal microscopy.
Arndt, M. A., Krauss, J., Schwarzenbacher, R., Vu, B. K., Greene, S., & Rybak, S. M. (2003). Generation of a highly stable, internalizing anti‐CD22 single‐chain Fv fragment for targeting non‐Hodgkin's lymphoma. International journal of cancer, 107(5), 822-829.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
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MOB-1248z-S(P) | Recombinant Anti-Human CD22 Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
HPAB-0709-FY-F(E) | Mouse Anti-CD22 Recombinant Antibody (clone AB008); scFv Fragment | ELISA | Mouse scFv |
HPAB-0370-FY-S(P) | Human Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-0370-FY-S(P)) | ELISA | Humanized scFv |
HPAB-0371-FY-S(P) | Human Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-0371-FY-S(P)) | Inhib | Humanized scFv |
HPAB-2052-FY-S(P) | Human Anti-CD22 Recombinant Antibody (clone B28); scFv Fragment | FC | Human scFv |
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