This product is a recombinant chimpanzee anti-HIV-1 monoclonal antibody. C108G specifically binds to gp120 and can be potentially used in the treatment of acquired immunodeficiency syndrome (AIDS), a condition in humans characterized by clinical features including wasting syndromes, central nervous system degeneration and profound immunosuppression that results in life-threatening opportunistic infections and malignancies.
Figure 1 Effects of reduction and deglycosylation on stability of epitopes in the V2 and other domains.
Recombinant BaL gp120 was captured on wells of a 96-well ELISA plate either untreated (open squares) or after deglycosylation with peptide N-glycosidase F (PNGase F; closed diamonds) or reduction by DTT (closed triangles). The reactivity of the indicated MAbs to control or treated rgp120 was titrated for 1 h at 37°C and bound antibody detected with alkaline phosphatase-conjugated anti-IgG, followed by incubation with alkaline phosphatase substrate. OD405, optical density at 405 nm.
The C108g Epitope in the V2 Domain of gp120 Functions as a Potent Neutralization Target When Introduced into Envelope Proteins Derived from Human Immunodeficiency Virus Type 1 Primary Isolates
Figure 2 Effects of mutations in the JR-FL V2 domain on neutralization by V2-specific MAbs C108g and 10/76b and MAbs to the V3 region (4117C) and CD4-binding domain (5145A).
Neutralization assays were performed with env deleted, luc-expressing NL4-3 provirus pseudotypes with either the parental JR-FL Env (closed triangles) or the JR(GKV) variant expressing the C108g epitope in the JR-FL background (open triangles).
The C108g Epitope in the V2 Domain of gp120 Functions as a Potent Neutralization Target When Introduced into Envelope Proteins Derived from Human Immunodeficiency Virus Type 1 Primary Isolates
Figure 3 Analysis of neutralizing activities of MAbs against various domains for the viruses pseudotyped with the two parental Envs SF162 (closed circles) and JR-FL (closed triangles) and with the two C108g-expressing variants SF(NI GKV) (open circles) and JR(GKV) (open triangles).
The MAb used and the domain it recognizes are indicated at the top of each panel.
The C108g Epitope in the V2 Domain of gp120 Functions as a Potent Neutralization Target When Introduced into Envelope Proteins Derived from Human Immunodeficiency Virus Type 1 Primary Isolates
Figure 4 Fluorescence-activated cell sorter assays of the inhibition of binding of either gp120 to U87 cells expressing CD4 by MAbs or sCD4.
Env proteins were used at 1.0 g/ml, MAbs at 20 g/ml, and sCD4 at 100 g/ml. Binding of Env proteins to cells was detected by subsequent staining with biotinylated HIVIG, followed by R-phycoerythrin-conjugated streptavidin and quantitated on a Becton-Dickinson FACScalibur flow cytometer. The MAb used and the extent of inhibition compared to the control in the absence of antibody, measured as the decrease in mean fluorescence intensity compared to the control, are indicated in each panel.
The C108g Epitope in the V2 Domain of gp120 Functions as a Potent Neutralization Target When Introduced into Envelope Proteins Derived from Human Immunodeficiency Virus Type 1 Primary Isolates
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
PABL-135 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PGT135) | ELISA, Neut | Human IgG |
PABL-142 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 412d) | Neut, WB, ELISA, FuncS | Human IgG |
PABL-155 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC03) | WB, ELISA, Neut, FuncS | Human IgG |
PABL-156 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC-CH31) | WB, ELISA, Neut, FuncS | Human IgG |
PABL-160 | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 59.1) | WB, ELISA, FuncS | Mouse IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-135 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PGT135); Fab Fragment | ELISA, Neut | Human Fab |
PFBL-142 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 412d); Fab Fragment | WB, ELISA, FuncS | Human Fab |
PFBL-155 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC03); Fab Fragment | WB, ELISA, Neut, FuncS | Human Fab |
PSBW-160 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone F105); scFv Fragment | FC, Neut | Human scFv |
PSBW-178 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone b12); scFv Fragment | Neut, ELISA | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBZ-209 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 19.3H-L3); Fab Fragment | Neut | Human Fab |
MRO-3374CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone CC6B5); scFv Fragment | ELISA, FC | Human scFv |
MRO-3567CQ-S(P) | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone Chessie 13-39.1 (C13)); scFv Fragment | ELISA | Mouse scFv |
MRO-3568CQ-S(P) | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone D24); scFv Fragment | ELISA, Block | Mouse scFv |
MRO-3569CQ-S(P) | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone D25); scFv Fragment | ELISA, Block | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-028LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (MRO-028LC-VHH) | ELISA | Single domain antibody |
MRO-029LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (MRO-029LC-VHH) | ELISA | Single domain antibody |
MRO-030LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (MRO-030LC-VHH) | ELISA | Single domain antibody |
MRO-031LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (MRO-031LC-VHH) | ELISA | Single domain antibody |
MRO-032LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (MRO-032LC-VHH) | ELISA | Single domain antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-135LC | Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) | ELISA, WB, Neut | Chimeric antibody (mouse/human) |
MRO-135LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
MRO-135LC-F(E) | Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-155LC | Anti-HIV-1 gp120 Recombinant Antibody (GP44) | ELISA | Human antibody |
MRO-156LC | Anti-HIV-1 gp120 Recombinant Antibody (GP68) | ELISA, Inhib | Human antibody |
MRO-157LC | Anti-HIV-1 gp120 Recombinant Antibody (MN215) | ELISA | Human antibody |
MRO-161LC | Human Anti-HIV-1 gp120 Recombinant Antibody (MRO-161LC) | ELISA | Human IgG1, κ |
MRO-162LC | Human Anti-HIV-1 gp120 Recombinant Antibody (MRO-162LC) | ELISA, FC, Neut, SPR | Human IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-041LC-F(E) | Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (D59/A2) | ELISA | Mouse antibody |
MRO-042LC-F(E) | Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (F58/H3) | ELISA | Mouse antibody |
MRO-043LC-F(E) | Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (P1/D12) | ELISA | Mouse antibody |
MRO-044LC-F(E) | Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (P4/D10) | ELISA | Mouse antibody |
MRO-045LC-F(E) | Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (A47/B1) | ELISA | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4220 | Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) | WB, ELISA | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-101-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment | WB, ELISA, FuncS | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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