Recombinant Human Antibody (F425-B4e8) is capable of binding to HIV-1 gp120, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV-1 gp120 mAb and CH1-3 region of human IgG2 and a light chain (LC) encoding VL from anti-HIV-1 gp120 mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. Human mAb B4e8 neutralizes a subset of viruses from HIV-1 subtypes B, C and D.
Figure 1 Competition of PG9 with antibodies against the V2 loop (10/76b), V3 loop (F425/b4e8), and the CD4i site (X5) for gp120 binding.
Antigens were coated directly onto ELISA wells in the experiments shown, but similar results were also obtained when antigens were captured by antibodies against noncompetitive epitopes.
Walker, L. M., Phogat, S. K., Chan-Hui, P. Y., Wagner, D., Phung, P., Goss, J. L., ... & Lehrman, J. K. (2009). Broad and potent neutralizing antibodies from an African donor reveal a new HIV-1 vaccine target. Science, 326(5950), 285-289.
Figure 2 Comparison of the neutralization breadth of mAbs F425-B4e8 and 447-52D
IC₅₀ values below 50 μg/ml are denoted in bold type.
Pantophlet, R., Aguilar-Sino, R. O., Wrin, T., Cavacini, L. A., & Burton, D. R. (2007). Analysis of the neutralization breadth of the anti-V3 antibody F425-B4e8 and re-assessment of its epitope fine specificity by scanning mutagenesis. Virology, 364(2), 441-453.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
NABG-082 | Recombinant Anti-HIV-1 gp120 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
MRO-028LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91C2) | ELISA | Single domain antibody |
MRO-029LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91F10) | ELISA | Single domain antibody |
MRO-030LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91E1) | ELISA | Single domain antibody |
MRO-031LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91B5) | ELISA | Single domain antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBZ-164 | Human Anti-HIV-1 gp120 Recombinant Antibody; scFv Fragment (PSBZ-164) | Neut | Human scFv |
PSBZ-165 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone GL-VRC01); scFv Fragment | Neut | Human scFv |
PSBZ-209 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 19.3H-L3); scFv Fragment | Neut | Human scFv |
PSBZ-210 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 19.3H-L1); scFv Fragment | Neut | Human scFv |
PSBW-160 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone F105); scFv Fragment | FC, Neut | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-125LC | Anti-HIV-1 gp120 Recombinant Antibody (N6) | Neut, ELISA | Human antibody |
MRO-130LC | Human Anti-HIV-1 gp120 Recombinant Antibody (MRO-130LC) | ELISA, Neut, SPR | Human IgG |
MRO-136LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900973) | ELISA | Human antibody |
MRO-137LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900972) | ELISA | Human antibody |
MRO-138LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900990) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-135LC | Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) | ELISA, WB, Neut | Chimeric antibody (mouse/human) |
MRO-135LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
MRO-135LC-F(E) | Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-041LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (D59/A2) | ELISA | Mouse antibody |
MRO-042LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (F58/H3) | ELISA | Mouse antibody |
MRO-043LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (P1/D12) | ELISA | Mouse antibody |
MRO-044LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (P4/D10) | ELISA | Mouse antibody |
MRO-045LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (A47/B1) | ELISA | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4220 | Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) | WB, ELISA | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-3132CQ | Human Anti-HIV-1 gp120 Recombinant Antibody (clone CH40) | ELISA, Block | Human IgG1 |
MRO-3133CQ | Human Anti-HIV-1 gp120 Recombinant Antibody (clone CH48) | Neut | Human IgG1 |
MRO-3134CQ | Human Anti-HIV-1 gp120 Recombinant Antibody (clone CH49) | ELISA | Human IgG1 |
MRO-3135CQ | Human Anti-HIV-1 gp120 Recombinant Antibody (clone CH51) | ELISA, Block | Human IgG1 |
MRO-3136CQ | Human Anti-HIV-1 gp120 Recombinant Antibody (clone CH52) | ELISA, Block | Human IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-3191CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone m16); scFv Fragment | Neut | Human scFv |
MRO-3192CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone m22 (R-1)); scFv Fragment | Neut | Human scFv |
MRO-3193CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone m24 (R-2)); scFv Fragment | Neut | Human scFv |
MRO-3194CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone m36); scFv Fragment | ELISA, Neut | Human scFv |
MRO-3195CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone M90); scFv Fragment | ELISA | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-101-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment | WB, ELISA, FuncS | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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