Recombinant Human Antibody (VRC-PG04) is capable of binding to HIV-1 gp120, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV-1 gp120 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HIV-1 gp120 mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Figure 1 Binding affinity constants of VRC01, VRC-PG04, and IgG b12 to core and outer domain constructs as assessed using Octet Biolayer Interferometry.
Sequential truncations of the outer domain molecule resulted in lower binding affinity while structural stabilization, mutagenesis, and glycan removal led to increased affinity for VRC01 and VRC-PG04 antibodies.
Joyce, M. G., Kanekiyo, M., Xu, L., Biertümpfel, C., Boyington, J. C., Moquin, S.,... & Zhang, B. (2013). Outer domain of HIV-1 gp120: antigenic optimization, structural malleability, and crystal structure with antibody VRC-PG04.Journal of virology, 87(4), 2294-2306.
Figure 2 Surface plasmon resonance sensorgrams of VRC-PG04 antibody immobilized using an anti-Fc antibody and OD4.2.2 flowed over the respective antibody.
The black lines represent the best fit of the kinetic data to a 1:1 binding model. Experiments were carried out at 25°C in PBS buffer (pH 7.4). koff, on-rate constant (M−1s−1); kon, off-rate constant (s−1); KD, equilibrium dissociation constant.
Joyce, M. G., Kanekiyo, M., Xu, L., Biertümpfel, C., Boyington, J. C., Moquin, S.,... & Zhang, B. (2013). Outer domain of HIV-1 gp120: antigenic optimization, structural malleability, and crystal structure with antibody VRC-PG04.Journal of virology, 87(4), 2294-2306.
Figure 3 Calorimetry data for the titration of OD4.2.2 with VRC-PG04 antibody in PBS buffer (pH 7.4).
Measurements were carried out in triplicate with a representative titration result shown. The top panel shows raw data with the area under each spike proportional to the heat produced at each injection of VRC-PG04 Fab. The lower panel shows integrated areas normalized to the number of moles of VRC-PG04 Fab.
Joyce, M. G., Kanekiyo, M., Xu, L., Biertümpfel, C., Boyington, J. C., Moquin, S.,... & Zhang, B. (2013). Outer domain of HIV-1 gp120: antigenic optimization, structural malleability, and crystal structure with antibody VRC-PG04.Journal of virology, 87(4), 2294-2306.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
NABG-082 | Recombinant Anti-HIV-1 gp120 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
MRO-028LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (MRO-028LC-VHH) | ELISA | Single domain antibody |
MRO-029LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (MRO-029LC-VHH) | ELISA | Single domain antibody |
MRO-030LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (MRO-030LC-VHH) | ELISA | Single domain antibody |
MRO-031LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (MRO-031LC-VHH) | ELISA | Single domain antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
PABL-520 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 44-VRC13.01) | Neut | Human IgG |
PABL-521 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 45-46M2) | ELISA, Neut | Human IgG |
PABL-522 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 45-VRC01.H5.F-185917) | WB, FuncS | Human IgG |
PABL-523 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 8ANC131) | WB, FuncS | Human IgG |
PABL-524 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 8ANC134) | WB, IHC, IF, FuncS | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-005LC | Human Anti-HIV-1 gp120 Recombinant Antibody (MRO-005LC) | ELISA | Human IgG1 |
MRO-006LC | Human Anti-HIV-1 gp120 Recombinant Antibody (MRO-006LC) | ELISA | Human IgG1 |
MRO-007LC | Human Anti-HIV-1 gp120 Recombinant Antibody (MRO-007LC) | ELISA | Human IgG1 |
MRO-011LC | Human Anti-HIV-1 gp120 Recombinant Antibody (MRO-011LC) | ELISA | Human IgG1 |
MRO-012LC | Human Anti-HIV-1 gp120 Recombinant Antibody (MRO-012LC) | ELISA | Human IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-041LC | Anti-HIV-1 gp120 Recombinant Antibody (D59/A2) | ELISA | Mouse antibody |
MRO-042LC | Anti-HIV-1 gp120 Recombinant Antibody (F58/H3) | WB, ELISA | Mouse antibody |
MRO-043LC | Anti-HIV-1 gp120 Recombinant Antibody (P1/D12) | ELISA | Mouse antibody |
MRO-044LC | Anti-HIV-1 gp120 Recombinant Antibody (P4/D10) | ELISA | Mouse antibody |
MRO-045LC | Anti-HIV-1 gp120 Recombinant Antibody (A47/B1) | ELISA | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-135LC | Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) | ELISA, WB, Neut | Chimeric antibody (mouse/human) |
MRO-135LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
MRO-135LC-F(E) | Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4220 | Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) | WB, ELISA | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-3278CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 3224); Fab Fragment | Neut | Human Fab |
MRO-3279CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 2412); Fab Fragment | Neut | Human Fab |
MRO-3280CQ-F(E) | Recombinant Human Anti-HIV-1 gp120 Antibody Fab Fragment (3019) | ELISA, Neut | Fab |
MRO-3281CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 2182); Fab Fragment | Neut | Human Fab |
MRO-3282CQ-F(E) | Recombinant Human Anti-HIV-1 gp120 Antibody Fab Fragment (2456) | Neut | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-1990-FY-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 16c); scFv Fragment | WB | Human scFv |
HPAB-1991-FY-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 23e); scFv Fragment | WB | Human scFv |
HPAB-0716-YJ-S(P) | Monkey Anti-gp120 Recombinant Antibody (2S1H3); scFv Fragment | ELISA, IHC | Monkey scFv |
PSBS-0130 | Human Anti-HIV-1 gp120 Recombinant Antibody (Clone VRC01cHuGL2); scFv Fragment | ELISA, FuncS | Human scFv |
PABJ-0086-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone DH677.3); scFv Fragment | ELISA, ADCC | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-101-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment | WB, ELISA, FuncS | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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