Recombinant Human Antibody (D5) is capable of binding to HIV-1 gp41, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV-1 gp41 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HIV-1 gp41 mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Figure 1 HNP-1 synergizes with neutralizing antibodies to gp41 and with HIV serum in inhibiting HIV-1 fusion.
HXB2 pseudoviruses were pre-bound to human PBMCs in the cold, and fusion was initiated in the absence or in the presence of indicated antibodies by shifting to 37 u C. Data are normalized to the extent of fusion (measured by the BlaM assay) in the absence of defensin or antibodies (control). Addition of 24 m M HNP-1 in HBSS with 10% human serum did not affect HXB2 fusion. The following concentrations of antibodies were used: D5 (25 m g/ml), bF-3674 (12 m g/ml), 2F5 (2.5 m g/ml), PG9 (20 m g/ml). HIV-1 neutralizing serum was diluted 1:15 prior to adding to cells. Experiments were performed either in the absence (black bars) or in the presence (red bars) of 24 m M HNP-1 in 10% human serum. Experiment with 3 m M C52L were used as a negative control.
Demirkhanyan, L., Marin, M., Lu, W., & Melikyan, G. B. (2013). Sub-inhibitory concentrations of human α-defensin potentiate neutralizing antibodies against HIV-1 gp41 pre-hairpin intermediates in the presence of serum. PLoS pathogens, 9(6), e1003431.
Figure 2 Antiviral activity of D5.
(A) D5 scFv and IgG1 inhibit HIV entry in the HIVRP assay. The bluegreen fluorescence ratio is proportional to virus entry. X5 scFv is shown as a control. (B) D5 scFv and IgG1 inhibit HIV infectivity in a single-cycle infection assay. X5 scFv is shown as a control.
Miller, M. D., Geleziunas, R., Bianchi, E., Lennard, S., Hrin, R., Zhang, H., ... & Mattu, M. (2005). A human monoclonal antibody neutralizes diverse HIV-1 isolates by binding a critical gp41 epitope. Proceedings of the National Academy of Sciences, 102(41), 14759-14764.
Figure 2 Competition ELISA using IgGs D5 and 8K8, and Fab DN9 against their biotinylated (Bio) counterparts for recognition of immobilized 5-Helix.
Bio-8K8 IgG (left panel), Bio-D5 IgG (middle panel), and Bio-DN9 Fab (right panel) at concentrations previously determined to generate non-saturating binding signals were added to wells coated with immobilized 5-Helix that were pretreated with various concentrations of competing mAb. Results are plotted as a percentage of the maximal ELISA binding signal of the Bio-mAb in the absence of competitor.
Nelson, J. D., Kinkead, H., Brunel, F. M., Leaman, D., Jensen, R., Louis, J. M., ... & Dawson, P. E. (2008). Antibody elicited against the gp41 N-heptad repeat (NHR) coiled-coil can neutralize HIV-1 with modest potency but non-neutralizing antibodies also bind to NHR mimetics. Virology, 377(1), 170-183.
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CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-185 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 8062); Fab Fragment | WB, ELISA, FuncS | Human Fab |
PFBL-186 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 2F5); Fab Fragment | WB, Neut, FuncS | Human Fab |
PFBL-187 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 3BC176); Fab Fragment | Neut, FuncS | Human Fab |
PFBL-188 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 3BC315); Fab Fragment | Neut, FuncS | Human Fab |
PFBC-069 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone HK20); Fab Fragment | Neut, ELISA | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABC-069 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone HK20) | WB, ELISA, Neut | Human IgG |
MRO-842CQ | Mouse Anti-HIV-1 gp41 Recombinant Antibody (clone 1911) | WB | Mouse IgG2a |
MRO-843CQ | Mouse Anti-HIV-1 gp41 Recombinant Antibody (clone 191) | WB | Mouse IgG2 |
MRO-844CQ | Mouse Anti-HIV-1 gp41 Recombinant Antibody (clone 10E9) | WB, RIPA, ELISA, IF | Mouse IgG1 |
MRO-845CQ | Mouse Anti-HIV-1 gp41 Recombinant Antibody (clone 10F6) | ELISA, WB, IP, IF | Mouse IgG2b |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBZ-068 | Mouse Anti-HIV-1 gp41 Recombinant Antibody (clone 1583); scFv Fragment | Neut | Mouse scFv |
PSBW-068 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone D5); scFv Fragment | WB, ELISA | Human scFv |
PSBC-069 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone HK20); scFv Fragment | Neut, ELISA | Human scFv |
PSBS-0051 | Monkey Anti-HIV-1 gp41 Recombinant Antibody (Clone DH570.4); scFv Fragment | ELISA, Neut, FuncS | Monkey scFv |
PSBS-0052 | Monkey Anti-HIV-1 gp41 Recombinant Antibody (Clone DH570.9); scFv Fragment | ELISA, FuncS | Monkey scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-074LC | Anti-HIV-1 gp41 Recombinant Antibody (W) | ELISA | Human antibody |
MRO-077LC | Anti-HIV-1 gp41 Recombinant Antibody (3D6) | ELISA | Human antibody |
MRO-079LC | Anti-HIV-1 gp41 Recombinant Antibody (3D9) | ELISA | Human antibody |
MRO-093LC | Anti-HIV-1 gp41 Recombinant Antibody (clone 43) | ELISA | Human antibody |
MRO-025LC-S(P) | Anti-HIV-1 gp41 Recombinant Antibody scFv Fragment (4E10-IgG3) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-LC746 | A*2402/HIV-1 gp41 (RYLKDQQLL) MHC Tetramer | FCM |
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