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Recombinant Mouse anti-Human MBD1 Monoclonal antibody (2C7) (CAT#: MOB-1223CT)

Recombinant Mouse monoclonal antibody to Human MBD1, expressed in Chinese Hamster Ovary cells(CHO).


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  • Gene Expression
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Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Figure 1 IF staining of human cell line HaCaT
(Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68850/1715_A5_8_cr57fd2701ec147_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/923_F1_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/33413_A_2_1_selected.jpg
Figure 4 IHC staining of human urinary bladder
Figure 4 IHC staining of human urinary bladder
(Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36003/71834_A_5_5_selected.jpg
Figure 5 Colon
Figure 5 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/33413_A_7_3.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/33413_A_7_5.jpg
Figure 7 Testis
Figure 7 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/33413_A_6_6.jpg
Figure 8 Lymph node
Figure 8 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/33413_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141644-MBD1

Specifications

  • Immunogen
  • Recombinant fragment: HHLGPTLKPT LATRTAQPDH TQAPTKQEAG GGFVLPPPGT DLVFLREGAS SPVQVPGPVA ASTEALLQEA QCSGLSWVVA LPQVKQEKAD TQDE, corresponding to amino acids 415-508 of Human MBD1, with a 26 kDa proprietary tag.
  • Host Species
  • Mouse
  • Species Reactivity
  • Human
  • Clone
  • 2C7
  • Applications
  • Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Western Blot
  • Conjugate
  • Unconjugated

Product Property

  • Purity
  • Protein A purified
  • Format
  • Liquid
  • Concentration
  • 1.0 mg/ml
  • Buffer
  • Preservative: None Constituents: 1X PBS, pH 7.2
  • Storage
  • Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Target

  • Alternative Names
  • MBD1; methyl-CpG binding domain protein 1; RFT; PCM1; CXXC3; methyl-CpG-binding domain protein 1; CXXC-type zinc finger protein 3; protein containing methyl-CpG-binding domain 1; methyl-CpG binding domain protein 1 isoform PCM1; the regulator of fibroblast growth factor 2 (FGF-2) transcription;
  • Function
  • DNA binding; metal ion binding; methyl-CpG binding; protein binding; sequence-specific DNA binding transcription factor activity; transcription corepressor activity; zinc ion binding;

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Chicken IgY Antibody

CAT Product Name Application Type
BRD-0357MZ Chicken Anti-methyl-CpG binding domain protein Polyclonal IgY Indirect ELISA, WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2178 Hi-Affi™ Recombinant Rabbit Anti-MBD1 Monoclonal Antibody (DS2178AB) ICC/IF, IHC-P, IP, WB IgG

Recombinant Antibody

CAT Product Name Application Type
VS4-CJ30 Recombinant Rabbit Anti-MBD1 Antibody (clone 10B10) ELISA, IF Rabbit IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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