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Recombinant Rabbit Anti-MBD1 Antibody (clone 10B10) (CAT#: VS4-CJ30)

This rabbit anti-MBD1 antibody (clone 10B10) is a product that recognizes human MBD1. The immunogen of this antibody is a synthesized peptide derived from human MBD1. The mAb has been tested for use in ELISA, Immunofluorescence (1:50-1:200).


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Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Figure 1 IF staining of human cell line HaCaT
(Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68850/1715_A5_8_cr57fd2701ec147_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/923_F1_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/33413_A_2_1_selected.jpg
Figure 4 IHC staining of human urinary bladder
Figure 4 IHC staining of human urinary bladder
(Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36003/71834_A_5_5_selected.jpg
Figure 5 Colon
Figure 5 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/33413_A_7_3.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/33413_A_7_5.jpg
Figure 7 Testis
Figure 7 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/33413_A_6_6.jpg
Figure 8 Lymph node
Figure 8 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/33413_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141644-MBD1

Specifications

  • Immunogen
  • A synthesized peptide derived from human MBD1
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human MBD1
  • Species Reactivity
  • Human
  • Clone
  • 10B10
  • Applications
  • ELISA, IF
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Affinity chromatography
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.
  • Preservative
  • 0.02% Sodium Azide
  • Storage
  • Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

Applications

  • Application Notes
  • This antibody has been tested for use in ELISA, Immunofluorescence (1:50-1:200).

Target

  • Alternative Names
  • CXXC-type zinc finger protein 3; Methyl-CpG-binding protein MBD1; Protein containing methyl-CpG-binding domain 1; CXXC3; PCM1; RFT
  • Long Name
  • Methyl-CpG binding domain protein 1
  • Cellular Localization
  • Chromosome, Nucleus
  • Post Translation Modifications
  • Sumoylated, sumoylation may increase interaction with ATF7IP.
  • Function
  • Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting ATF7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Recombinant Antibody

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MOB-1223CT Recombinant Mouse anti-Human MBD1 Monoclonal antibody (2C7) ELISA, WB

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0357MZ Chicken Anti-methyl-CpG binding domain protein Polyclonal IgY Indirect ELISA, WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2178 Hi-Affi™ Recombinant Rabbit Anti-MBD1 Monoclonal Antibody (DS2178AB) ICC/IF, IHC-P, IP, WB IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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