Recombinant Mouse anti-Human RNF20 Monoclonal antibody (SOG30-96) (CAT#: MOB-1045CT)
Recombinant Mouse monoclonal antibody to Human RNF20, expressed in Chinese Hamster Ovary cells(CHO).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51773/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51773/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/if_selected.jpg
Figure 3 IHC staining of human gall bladder
(Immunohistochemical staining of human gall bladder shows distinct nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/ihc_selected.jpg
(Immunohistochemical staining of human gall bladder shows distinct nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/ihc_selected.jpg
Figure 4 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_B_8_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_B_8_5.jpg
Figure 5 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_A_7_3.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_A_7_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_A_7_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_A_6_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_A_6_6.jpg
Figure 8 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_A_9_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000155827-RNF20
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000155827-RNF20
Specifications
- Immunogen
- Synthetic peptide: SEDLSSQSSASKASQEDANEIKSKRD, corresponding to amino acids 540-565 of Human RNF20
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- SOG30-96
- Applications
- Used for immunoassay techniques such as: I-Enzyme-linked Immunosorbent Assay; Immunocytochemistry/Immunofluorescence; Western Blot
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Concentration
- 2.3 mg/ml
- Buffer
- Preservative: 15mM Sodium Azide Constituents: 0.01M PBS, pH 7.4
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- RNF20; ring finger protein 20, E3 ubiquitin protein ligase; BRE1; BRE1A; hBRE1; E3 ubiquitin-protein ligase BRE1A; BRE1-A; homolog of S. cerevisiae BRE1; BRE1 E3 ubiquitin ligase homolog;
- Gene ID
- 56254
- UniProt ID
- Q5VTR2
- Protein Refseq
- NP_062538
- Function
- histone binding; ligase activity; metal ion binding; p53 binding; protein binding; transcription coactivator activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; contributes_to ubiquitin-protein ligase activity; zinc ion binding;
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3085 | Hi-Affi™ Recombinant Rabbit Anti-RNF20 Monoclonal Antibody (DS3085AB) | ICC/IF, WB, FC, IP | IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1341-CN | Recombinant Rabbit Anti-RNF20 Monoclonal Antibody (CBACN-486) | WB, IP, FC | Rabbit IgG |
| ZG-0098U | Rabbit Anti-RNF20 Recombinant Antibody (clone 2H12) | ELISA, WB, IHC, IF | Rabbit IgG |
| VS3-WK1029 | Mouse Anti-RNF20 Recombinant Antibody (VS3-WK1029) | WB | Mouse IgG |
| VS3-WK1030 | Rabbit Anti-RNF20 Recombinant Antibody (VS3-WK1030) | WB, IP, FC | Rabbit IgG |
| VS3-XY3487 | Rabbit Anti-RNF20 Recombinant Antibody (clone R09-2F6) | WB, ICC, IF, IP, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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