The purified Rabbit Anti-RNF20 Recombinant Antibody (clone 2H12) displayed high specificity against the RNF20 protein of human.
Figure 1 Rabbit Anti-RNF20 Antibody (ZG-0098U) in WB
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate. All lanes: RNF20 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 114 kDa
Observed band size: 114 kDa
Figure 2 Rabbit Anti-RNF20 Antibody (ZG-0098U) in IHC
IHC image of ZG-0098U diluted at 1:100 and staining in paraffin-embedded human melanoma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Figure 3 Rabbit Anti-RNF20 Antibody (ZG-0098U) in IF
Immunofluorescence staining of Hela Cells with ZG-0098U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1045CT | Recombinant Mouse anti-Human RNF20 Monoclonal antibody (SOG30-96) | I-ELISA, ICC/IF, WB | |
MRO-1341-CN | Recombinant Rabbit Anti-RNF20 Monoclonal Antibody (CBACN-486) | WB, IP, FC | Rabbit IgG |
VS3-WK1029 | Mouse Anti-RNF20 Recombinant Antibody (VS3-WK1029) | WB | Mouse IgG |
VS3-WK1030 | Rabbit Anti-RNF20 Recombinant Antibody (VS3-WK1030) | WB, IP, FC | Rabbit IgG |
VS3-XY3487 | Rabbit Anti-RNF20 Recombinant Antibody (clone R09-2F6) | WB, ICC, IF, IP, FC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-3085 | Hi-Affi™ Recombinant Rabbit Anti-RNF20 Monoclonal Antibody (DS3085AB) | ICC/IF, WB, FC, IP | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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