Anti-Human MADCAM1 Recombinant Antibody (PF-00547659) (CAT#: TAB-509CL)
Recombinant Humanized monoclonal antibody expressed in CHO binding to Human MADCAM1. PF-00547659 is an anti-human MAdCAM monoclonal antibody which has potential utility in the treatment of inflammatory conditions by blocking tissue homing of activated α4β7+ leukocytes. The characterization of a rodent cross-reacting antibody as a surrogate for PF-00547659 in the search for potential pharmacological biomarkers and the determination of efficacious doses was effective in addressing the restricted orthologous cross-reactivity of PF-00547659 and the challenges this poses with respect to efficacy and safety testing.
Specific Inquiry
IHC
Figure 1 Immunohistochemical staining of endothelium expressing MAdCAM in human colon.
PF-00547659 staining, arrows indicate specific endothelial staining in the gut lamina propria. The bar represents 200 mm. MAdCAM, mucosal addressin cell adhesion molecule.
Pullen, N., Molloy, E., Carter, D., Syntin, P., Clemo, F., Finco‐Kent, D., ... & Sreckovic, S. (2009). Pharmacological characterization of PF‐00547659, an anti‐human MAdCAM monoclonal antibody. British journal of pharmacology, 157(2), 281-293.
FuncS
Figure 2 Mean serum concentration of PF-00547659 following increasing i.v. doses in cynomolgus macaques.
Data are plotted as means ±SD (n = 4). Pharmacokinetic data from both males and females are represented together as a single mean.
Pullen, N., Molloy, E., Carter, D., Syntin, P., Clemo, F., Finco‐Kent, D., ... & Sreckovic, S. (2009). Pharmacological characterization of PF‐00547659, an anti‐human MAdCAM monoclonal antibody. British journal of pharmacology, 157(2), 281-293.
Specifications
- Derivation
- Humanized
- Type
- Antibody
- Species Reactivity
- Human
- Applications
- IHC, FuncS
- Related Disease
- Crohn's Disease; Inflammatory Diseases; Ulcerative Colitis
Product Property
- Purity
- 90–95% (SDS-PAGE)
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Functional Assay and Immunohistochemistry. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- MADCAM1; mucosal vascular addressin cell adhesion molecule 1; MACAM1; mucosal addressin cell adhesion molecule 1; MAdCAM-1; hMAdCAM-1; mucosal addressin cell adhesion molecule-1
- Gene ID
- 8174
- UniProt ID
- Q13477
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "MADCAM1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1935z | Mouse Anti-MADCAM1 Recombinant Antibody (MOB-1935z) | IHC, IF, ELISA | Mouse IgG2a |
| PABL-640 | Mouse Anti-MADCAM1 Recombinant Antibody (clone 10G3) | WB, IF, FuncS | Mouse IgG |
| PABL-641 | Human Anti-MADCAM1 Recombinant Antibody (clone PF-547659) | WB, IF, FuncS | Human IgG |
| MOB-1116CT | Recombinant Mouse anti-Human MADCAM1 Monoclonal antibody (28G6) | FC | |
| VS4-CJ24 | Recombinant Rabbit Anti-MADCAM1 Antibody (clone 12B5) | ELISA, FC | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1935z-F(E) | Recombinant Anti-Human MADCAM1 Antibody Fab Fragment | IF, Neut, FuncS | Fab |
| PFBL-635 | Mouse Anti-MADCAM1 Recombinant Antibody (clone 10G3); Fab Fragment | WB, IF, FuncS | Mouse Fab |
| PFBL-636 | Human Anti-MADCAM1 Recombinant Antibody (clone PF-547659); Fab Fragment | WB, IF, FuncS | Human Fab |
| HPAB-0040-DJB-F(E) | Human Anti-MADCAM1 Recombinant Antibody; Fab Fragment (HPAB-0040-DJB-F(E)) | ELISA | Human Fab |
| NS-051CN-F(E) | Human Anti-MADCAM1 Recombinant Antibody; Fab Fragment (NS-051CN-F(E)) | ELISA, WB, IHC, FC | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-635 | Mouse Anti-MADCAM1 Recombinant Antibody (clone 10G3); scFv Fragment | WB, IF, FuncS | Mouse scFv |
| PSBL-636 | Human Anti-MADCAM1 Recombinant Antibody (clone PF-547659); scFv Fragment | WB, IF, FuncS | Human scFv |
| NS-051CN-S(P) | Human Anti-MADCAM1 Recombinant Antibody; scFv Fragment (NS-051CN-S(P)) | ELISA, WB, IHC, FC | Human scFv |
| HPAB-0178-FY-S(P) | Human Anti-MADCAM1 Recombinant Antibody (clone 7.16.6_S); scFv Fragment | FC | Human scFv |
| HPAB-692-FY-S(P) | Mouse Anti-MADCAM1 Recombinant Antibody; scFv Fragment (HPAB-692-FY-S(P)) | ELISA | Mouse scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0714CL | Anti-Mouse Madcam1 Recombinant Antibody (MECA-367) | ELISA | |
| TAB-0714CL-S(P) | Anti-Mouse Madcam1 Recombinant Antibody scFv Fragment (MECA-367) | ELISA | |
| TAB-0714CL-F(E) | Anti-Mouse Madcam1 Recombinant Antibody Fab Fragment (MECA-367) | ELISA |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1715CQ | Recombinant Rat Anti-Madcam1 Antibody (11A55) | BL, WB | IgG2 |
| NEUT-1717CQ | Recombinant Mouse Anti-Madcam1 Antibody (CBL350) | BL, WB | IgG2a |
| NEUT-1718CQ | Recombinant Rat Anti-Madcam1 Antibody (MAB0842) | BL, FC, IHC-Fr, IP | IgG2 |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1716CQ | Recombinant Rat Anti-Madcam1 Antibody (CBL142) | Neut, WB | IgG2 |
| NEUT-1719CQ | Recombinant Rat Anti-Madcam1 Antibody (RM0284-11A55) | WB, Neut | IgG2 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2118 | Hi-Affi™ Recombinant Rabbit Anti-MADCAM1 Monoclonal Antibody (DS2118AB) | WB | IgG |
Neuroscience Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-051CN | Human Anti-MADCAM1 Recombinant Antibody (NS-051CN) | ELISA, WB, IHC, FC, Block, FuncS | Human IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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