Mouse Anti-Aβ Recombinant Antibody (clone AF1) (CAT#: VS-0623-WK72)
The antibody clone AF1 was produced by immunizing mouse with Peptides Aβ42. It can be used for detection of human Aβ in ELISA, DB and WB assays.
Specific Inquiry
DB
Figure 1 Immunoblot analysis of the conformational and sequence specificity of monovalent and bivalent AF1 antibodies.
Dilutions of Aβ, tau, and α-synuclein (αSyn) (disaggregated (D) and fibril (F)) were deposited on nitrocellulose membranes. Binding of monovalent (scFv) and bivalent AF1 (scFv-Fc) antibodies were evaluated at 100 nm (scFv) and 10 nm (scFv-Fc) in 5% milk.
WB
Figure 2 Western blot analysis of the conformational and sequence specificity of the AF1 antibody.
Aβ, tau, and α-synuclein (αSyn) (disaggregated (D) and fibril (F); 0.85 μg) were separated via SDS-PAGE and transferred to nitrocellulose membranes. The AF1-Fc antibody was used at 10 nm (1% milk).
FC
Figure 3 Bivalent AF1 antibodies display high conformational specificity for Aβ fibrils by flow cytometry.
Conformational specificities of AF1 antibodies for Aβ fibrils relative to disaggregated Aβ were evaluated for two bivalent formats of AF1 (Fc fusion) relative to monovalent AF1.
Specifications
- Immunogen
- Peptides Aβ42
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human Aβ
- Species Reactivity
- Human
- Clone
- AF1
- Applications
- ELISA, DB, WB, ELISA, FC
- Conjugate
- Unconjugated
- Related Disease
- Alzheimer's disease
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No Preservative
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Flow cytometry: 10-1000 nM
Western blot: 100 nm (scFv) and 10 nm (scFv-Fc)
Target
- Alternative Names
- AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma; alpha-sAPP
- Gene ID
- 351
- UniProt ID
- P05067
- Long Name
- Amyloid beta precursor protein
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Aβ"
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-82-F(E) | Recombinant Anti-human Aβ Antibody Fab Fragment | IP, Neut, FuncS | Fab |
| MHH-82-F(E) | Recombinant Human Anti-human Aβ Antibody Fab Fragment | ELISA, WB, IP, FuncS | Fab |
| HPAB-0425-WJ-F(E) | Mouse Anti-Aβ Recombinant Antibody (clone 2E7); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0432-WJ-F(E) | Mouse Anti-Aβ Recombinant Antibody (clone 5D1); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0958WJ-F(E) | Human Anti-Aβ Recombinant Antibody; Fab Fragment (HPAB-0958WJ-F(E)) | ELISA | Humanized Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-82-S(P) | Recombinant Anti-human Aβ Antibody scFv Fragment | WB, FC, FuncS | scFv |
| MHH-82-S(P) | Recombinant Human Anti-human Aβ Antibody scFv Fragment | WB, IP, FuncS | scFv |
| HPAB-0425-WJ-S(P) | Mouse Anti-Aβ Recombinant Antibody (clone 2E7); scFv Fragment | ELISA | Mouse scFv |
| HPAB-0432-WJ-S(P) | Mouse Anti-Aβ Recombinant Antibody (clone 5D1); scFv Fragment | ELISA | Mouse scFv |
| HPAB-1135WJ-S(P) | Human Anti-Aβ Recombinant Antibody (clone h10D5); scFv Fragment | ELISA | Humanized scFv |
Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B052(A) | Recombinant Anti-human Aβ Intrabody [(D-Arg)9] | ELISA, IF, Neut, FuncS | scFv-(D-Arg)9 |
| IAB-B052(G) | Recombinant Anti-human Aβ Intrabody [+36 GFP] | ELISA, Neut, FuncS | scFv-(+36GFP) |
| IAB-B052(T) | Recombinant Anti-human Aβ Intrabody [Tat] | PCA, WB, FuncS | scFv-Tat |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-610CT | Mouse Anti-Aβ Recombinant Antibody (TAB-610CT) | WB, Neut, FuncS, IHC | Mouse IgG |
| TAB-610CT-S(P) | Mouse Anti-Aβ Recombinant Antibody; scFv Fragment (TAB-610CT-S(P)) | ELISA | Mouse scFv |
| TAB-610CT-F(E) | Mouse Anti-Aβ Recombinant Antibody; Fab Fragment (TAB-610CT-F(E)) | ELISA | Mouse Fab |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-160LC | Recombinant Anti-Human Aβ Antibody (Fc glycosylation/Sialylated) | ELISA | Mouse antibody |
| Gly-161LC | Recombinant Anti-Human Aβ Antibody (Fc glycosylation/Sialylated) | ELISA | Mouse antibody |
| Gly-162LC | Recombinant Anti-Human Aβ Antibody (Fc glycosylation/Sialylated) | ELISA | Mouse antibody |
Sialylated IgG Glycan
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-160LC-1 | Recombinant Anti-Human Aβ Antibody (Fc glycosylation/Sialylated) | ELISA | Mouse antibody |
| Gly-161LC-1 | Recombinant Anti-Human Aβ Antibody (Fc glycosylation/Sialylated) | ELISA | Mouse antibody |
| Gly-162LC-1 | Recombinant Anti-Human Aβ Antibody (Fc glycosylation/Sialylated) | ELISA | Mouse antibody |
Deglycosylated Antibody (Non-glycosylated IgGs)
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-168LC | Recombinant Anti-Human Aβ Antibody (Non-glycosylated) | ELISA | Mouse antibody |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0425-WJ | Mouse Anti-Aβ Recombinant Antibody (clone 2E7) | IB | Mouse IgG |
| HPAB-0432-WJ | Mouse Anti-Aβ Recombinant Antibody (clone 5D1) | ELISA | Mouse IgG |
| HPAB-0958WJ | Human Anti-Aβ Recombinant Antibody (HPAB-0958WJ) | ELISA | Humanized IgG |
| HPAB-0959WJ | Human Anti-Aβ Recombinant Antibody (HPAB-0959WJ) | ELISA | Humanized IgG |
| HPAB-1135WJ | Human Anti-Aβ Recombinant Antibody (clone h10D5) | ELISA | Humanized IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS-0623-WK72. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.