Mouse Anti-ABCB1 Recombinant Antibody (clone MM4.17) (CAT#: HPAB-0076-YJ)

Figure 1 Reactivity of CEMʳᵉᵛ cells and their derivative variants with MAbs to P‐gp and CD4 T‐cell determinant.

Fluorescence profiles (solid line) measure the binding of the specific MAbs MM4.17 and CD4 to living/intact cells. Binding of an irrelevant immunoglobulin is also shown (dotted profiles).

Dupuis, M. L., Flego, M., Molinari, A., & Cianfriglia, M. (2003). Saquinavir induces stable and functional expression of the multidrug transporter P‐glycoprotein in human CD4 T‐lymphoblastoid CEMrev cells. HIV medicine, 4(4), 338-345.

Figure 2 Confocal microscopy.

MAb MM4.17 reactivity was mainly distributed as fluorescent‐green spots at the plasma membrane level of CEMvbl10, CEMsaq10 and CEMsaq15 cells. This pattern is consistent with P‐gp localization on MDR cells. By contrast, CEMrev cells were not stained with MAb MM4.17. In the insert, the arrowed CEMsaq10 cell is also shown at higher magnification.

Dupuis, M. L., Flego, M., Molinari, A., & Cianfriglia, M. (2003). Saquinavir induces stable and functional expression of the multidrug transporter P‐glycoprotein in human CD4 T‐lymphoblastoid CEMrev cells. HIV medicine, 4(4), 338-345.

Figure 3 Pgp plasma membrane localization in MDR osteosarcoma and co‐localization with endogenous full length ezrin.

Panel (a), Flow cytometry analysis of membrane Pgp expression in SaOS2, SaOS2R and SaOS2RΔ146 using the monoclonal antibody MM 4.17. The staining was performed with isotype control and is shown in black while Pgp antibody staining is shown in green. Panel (b), Confocal laser scanning microscopy (CLSM) analysis of Pgp distribution on the plasma membrane of MDR cells compared to SaOS2RΔ146‐transfected cell line. Upper panel: Pgp detection in MDR cells by CLSM microscopy (three‐dimensional reconstruction) on the SaOS2R plasma membrane. Endogenous ezrin is detected subsequently to Pgp staining by permeabilization of cells. In yellow, the areas of co‐localization (overlay). Middle panels: SaOS2RΔ146 cells lack plasma membrane Pgp expression. Lower panels: SaOS2RΔ146 cells show cytoplasmatic Pgp expression. Bar scale: 15 μm.

Brambilla, D., Zamboni, S., Federici, C., Lugini, L., Lozupone, F., Milito, A. D., ... & Fais, S. (2012). P‐glycoprotein binds to ezrin at amino acid residues 149–242 in the FERM domain and plays a key role in the multidrug resistance of human osteosarcoma. International journal of cancer, 130(12), 2824-2834.

Figure 4 Confocal laser scanning microscopy (CLSM) analysis of Pgp distribution on the plasma membrane of MDR cells compared to SaOS2RD146-transfected cell line.

Brambilla, D., Zamboni, S., Federici, C., Lugini, L., Lozupone, F., Milito, A. D., ... & Fais, S. (2012). P‐glycoprotein binds to ezrin at amino acid residues 149–242 in the FERM domain and plays a key role in the multidrug resistance of human osteosarcoma. International journal of cancer, 130(12), 2824-2834.