Human Anti-AChR Recombinant Antibody (clone 637) (CAT#: FAMAB-0002WJ)

Figure 1 Recombinant IgG1-131 binds fetal AChR and γ-subunit with high specificity and apparent affinity.

125I-α-BT blocking was measured as percentage inhibition in the 125I-α-BT binding to AChRs after co-incubating the individual antibody/AMC serum and 125I-α-BT labelled AChR.

Saxena, A., Stevens, J., Cetin, H., Koneczny, I., Webster, R., Lazaridis, K., ... & Molenaar, P. C. (2017). Characterization of an anti-fetal AChR monoclonal antibody isolated from a myasthenia gravis patient. Scientific reports, 7(1), 1-12.

Figure 2 Recombinant IgG1-131 binds fetal AChR and γ-subunit with high specificity and apparent affinity.

Analysis of competition between IgG1-131 and IgG1-637. IgG1-131 (100 nM) when co-incubated with IgG1-637 (25 nM), reduced the amount of 125I-α-BT co-precipitated with fetal AChR compared to 25 nM IgG1-637 alone.

Saxena, A., Stevens, J., Cetin, H., Koneczny, I., Webster, R., Lazaridis, K., ... & Molenaar, P. C. (2017). Characterization of an anti-fetal AChR monoclonal antibody isolated from a myasthenia gravis patient. Scientific reports, 7(1), 1-12.

Figure 3 IgG1-637 bind to human RMS cell lines.

Binding of IgG1-637 to cell surface fetal AChR on different RMS cell lines was analyzed by immunofluorescence. Binding of antibody was detected by goat anti-human IgG-Alexa 488 antibody (green) and Hoechst was used to stain the nuclei.

Saxena, A., Stevens, J., Cetin, H., Koneczny, I., Webster, R., Lazaridis, K., ... & Molenaar, P. C. (2017). Characterization of an anti-fetal AChR monoclonal antibody isolated from a myasthenia gravis patient. Scientific reports, 7(1), 1-12.

Figure 4 IgG1-131 and IgG1-637 bind to human RMS cell lines.

Analysis of 125I-α-BT bound to cell surface AChR on RMS cell lines after incubation with IgG1-131 or IgG1 637.

Saxena, A., Stevens, J., Cetin, H., Koneczny, I., Webster, R., Lazaridis, K., ... & Molenaar, P. C. (2017). Characterization of an anti-fetal AChR monoclonal antibody isolated from a myasthenia gravis patient. Scientific reports, 7(1), 1-12.

Figure 5 IgG1-131 does not lead to modulation of fetal AChR on TE671 cells, but partly displaces 125I-α-BT.

The effect of IgG1-637 on the 125I-α-BT binding to TE671 (fetal AChR) and CN21 (adult AChR) cells is shown as normalized cell bound radioactivity at varying molar concentrations of the antibodies. Cells were incubated with antibodies for 3 h and subsequently labelled with 125I-α-BT.

Saxena, A., Stevens, J., Cetin, H., Koneczny, I., Webster, R., Lazaridis, K., ... & Molenaar, P. C. (2017). Characterization of an anti-fetal AChR monoclonal antibody isolated from a myasthenia gravis patient. Scientific reports, 7(1), 1-12.