Rabbit Anti-AKT1 Recombinant Antibody (clone 4B6) (CAT#: ZG-0540J)

Figure 1 Rabbit Anti-AKT1 Antibody (ZG-0540J) in WB

Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse brain tissue, Rat heart tissue
All lanes: AKT1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa

Figure 2 Rabbit Anti-AKT1 Antibody (ZG-0540J) in IHC

IHC image of ZG-0540J diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Figure 3 Rabbit Anti-AKT1 Antibody (ZG-0540J) in IF

Immunofluorescence staining of Hela Cells with ZG-0540J at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).