Human Anti-APS Recombinant Antibody (clone IS1) (CAT#: HPAB-1958-FY)

Figure 1 All monoclonal aCL require serum co-factors in their binding to CL.

All test antibodies were used at 1 mg/ml, except for CL15 and CL24 which were used at 2 mg/ml, which had been predetermined to be optimal for each aCL.

Zhu, M. , Olee, T. , Le, D. T. , Roubey, R. A. S. , Hahn, B. H. , & Woods, J. V. L. , et al. (1999). Characterization of igg monoclonal anti-cardiolipin/anti-beta2gp1 antibodies from two patients with antiphospholipid syndrome reveals three species of antibodies. British Journal of Haematology, 105(1), 102-109.

Figure 2 Binding properties of human IgG monoclonal aCL.

Monoclonal aCL were analysed comparatively for their binding to CL/ bovine serum and four unrelated antigens, including chicken ovalbumin (OVA), type VI collagen, single-stranded DNA (ssDNA) and KLH.

Zhu, M. , Olee, T. , Le, D. T. , Roubey, R. A. S. , Hahn, B. H. , & Woods, J. V. L. , et al. (1999). Characterization of igg monoclonal anti-cardiolipin/anti-beta2gp1 antibodies from two patients with antiphospholipid syndrome reveals three species of antibodies. British Journal of Haematology, 105(1), 102-109.

Figure 3 Effect of anti-β2-glycoprotein I (anti-β2GPI) IgG on the release of neutrophil extracellular traps (NETs) by neutrophils.

NET release from neutrophils stimulated with the indicated antibodies. Control neutrophils were treated with purified monoclonal antiphospholipid antibodies (aPL; 10 mg/ml) for 3 hours in the presence or absence of 10% autologous serum. IS4, aPL, CL1, and CL24 are known to bind b2GPI, while IS1 and IS2 do not.

Yalavarthi, S., Gould, T. J., Rao, A. N., Mazza, L. F., Morris, A. E., Núñez‐Álvarez, C., ... & Knight, J. S. (2015). Release of neutrophil extracellular traps by neutrophils stimulated with antiphospholipid antibodies: a newly identified mechanism of thrombosis in the antiphospholipid syndrome. Arthritis & rheumatology, 67(11), 2990-3003.