Mouse Anti-AQP4 Recombinant Antibody (VS3-CJ1042) (CAT#: VS3-CJ1042)

• Immunogen
• KLH-conjugated synthetic peptide encompassing a sequence of human Aquaporin 4

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human, Mouse, Rat AQP4

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, ICC, IF, IHC

• Conjugate
• Unconjugated

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 30% Glycerol, 0.87% NaCl, 0.42% Potassium phosphate, pH7.3.

• Preservative
• 0.01% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipped at 4°C

• Application Notes
• This antibody has been tested for use in Western Blot (1:1000-1:3000), Immunocytochemistry/Immunofluorescence (1:100-1:200), Immunohistochemistry (1:50-1:200).

• Alternative Names
• MIWC; WCH4; Haqp4

• Gene ID
• 361

• UniProt ID
• P55087

• Sequence Similarities
• Belongs to the MIP/aquaporin (TC 1.A.8) family.

• Cellular Localization
• Cell membrane, Cell projection, Endosome, Membrane

• Post Translation Modifications
• Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamate increases conductance by 40% (By similarity).
  Isoform 2: Palmitoylated on its N-terminal region. Isoform 1: Not palmitoylated.

• Protein Refseq
• NP_001641.1; NP_004019.1

• Function
• Forms a water-specific channel (PubMed:7559426, PubMed:8601457, PubMed:19383790).
  Plays an important role in brain water homeostasis and in glymphatic solute transport. Required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability (By similarity).

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody