Recombinant Mouse Anti-AQP4 Antibody (VS3-FY92) (CAT#: VS3-FY92)

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• The Aquaporin 4 Mouse Monoclonal antibody detects endogenous Aquaporin 4 proteins.

• Species Reactivity
• Human, Mouse, Rat

• Applications
• Western Blot, Immunohistochemistry, Immunofluorescence

• Conjugate
• Unconjugated

• MW
• 26 kDa

• Purification
• Affinity Purified

• Purity
• >95%

• Format
• Liquid

• Concentration
• 1 mg/mL

• Buffer
• PBS (without Mg^{2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% Glycerol}

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/1000-1/2000
  Immunofluorescence: 1/100-200
  Immunohistochemistry:1/50-300

• Alternative Names
• Aquaporin 4; Mercurial-Insensitive Water Channel; MIWC; WCH4; Aquaporin Type4; Aquaporin-4; AQP-4

• Gene ID
• 361

• UniProt ID
• P55087

• Sequence Similarities
• Belongs to the MIP/aquaporin (TC 1.A.8) family.

• Cellular Localization
• Cell membrane, Cell projection, Endosome, Membrane

• Post Translation Modifications
• Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamate increases conductance by 40% (By similarity).
  Isoform 2: Palmitoylated on its N-terminal region. Isoform 1: Not palmitoylated.

• Protein Refseq
• NP_001641.1; NP_004019.1

• Function
• Forms a water-specific channel (PubMed:7559426, PubMed:8601457, PubMed:19383790).
  Plays an important role in brain water homeostasis and in glymphatic solute transport. Required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability (By similarity).

Secondary Antibody

• 2ND-037CQ
• Chicken Anti-Mouse IgG (H&L) Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-041CQ
• Llama Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal