Rabbit Anti-ATF2 Recombinant Antibody (clone 3D12) (CAT#: ZG-0552J)
This product is a rabbit antibody that recognizes ATF2.
Specific Inquiry
WB
Figure 1 Rabbit Anti-ATF2 Antibody (ZG-0552J) in WB
Western Blot
Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate
All lanes: ATF2 antibody at 1.2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55, 36, 24, 49, 53, 14, 25, 16 KDa
Observed band size: 60 KDa
IHC
Figure 2 Rabbit Anti-ATF2 Antibody (ZG-0552J) in IHC
IHC image of ZG-0552J diluted at 1:115.5 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Specifications
- Immunogen
- A synthesized peptide derived from human ATF2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ATF2
- Species Reactivity
- Human
- Clone
- 3D12
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- HB16; CREB2; TREB7; CREB-2; CRE-BP1; cyclic AMP-dependent transcription factor ATF-2; activating transcription factor 2 splice variant ATF2-var2; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; cyclic AMP-responsive element-binding protein 2; histone acetyltransferase ATF2
- Gene ID
- 1386
- UniProt ID
- P15336
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ATF2"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0274 | Hi-Affi™ Rabbit Anti-ATF2 Recombinant Antibody (clone DS274AB) | IF, IP, WB | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0130-CN | Rabbit Anti-ATF2 Recombinant Antibody (clone CBACN-047) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-0315F | Mouse Anti-ATF2 Recombinant Antibody (ZG-0315F) | WB, IHC, ELISA | Mouse IgG |
| VS3-CJ672 | Rabbit Anti-ATF2 Recombinant Antibody (VS3-CJ672) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-XY99 | Mouse Anti-ATF2 Recombinant Antibody (clone 4F12) | ELISA, WB, IHC | Mouse IgG1 |
| VS3-FY102 | Recombinant Rabbit Anti-ATF2 Antibody (clone R09-8F4) | WB, ICC, IF, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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