Recombinant Rabbit Anti-ATF2 Antibody (clone R09-8F4) (CAT#: VS3-FY102)

• Immunogen
• A synthetic peptide of human ATF2

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human ATF2

• Species Reactivity
• Human

• Clone
• R09-8F4

• Applications
• Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

• Conjugate
• Unconjugated

• MW
• Calculated MW: 55 kDa; Observed MW: 70 kDa

• Purification
• Affinity Purified

• Purity
• >95%

• Format
• Liquid

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunofluorescence: 1/50-1/200
  Immunoprecipitation: 1/20

• Alternative Names
• Activating Transcription Factor 2; Cyclic AMP-Responsive Element-Binding Protein 2; CAMP Response Element-Binding Protein CRE-BP1; CAMP-Dependent Transcription Factor ATF-2; Histone Acetyltransferase ATF2; CREB-2; CREB2; HB16; Activating Transcription Factor 2 Splice Variant ATF2-Var2

• Gene ID
• 1386

• UniProt ID
• P15336

• Sequence Similarities
• Belongs to the bZIP family. ATF subfamily.

• Cellular Localization
• Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

• Post Translation Modifications
• Phosphorylation of Thr-69 by MAPK14 and MAPK11, and at Thr-71 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity (PubMed:9430721, PubMed:12110590).
  Phosphorylated by PLK3 following hyperosmotic stress (PubMed:21098032).
  Also phosphorylated and activated by JNK and CaMK4 (PubMed:8855261).
  ATM-mediated phosphorylation at Ser-490 and Ser-498 stimulates its function in DNA damage response (PubMed:15916964).
  Phosphorylation at Ser-62, Thr-73 and Ser-121 activates its transcriptional activity (PubMed:15105425).
  Phosphorylation at Thr-69 or Thr-71 enhances acetylation of histones H2B and H4 (PubMed:10821277).

• Protein Refseq
• NP_001243019.1; NP_001243020.1; NP_001243021.1

• Function
• Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro (PubMed:10821277).
  In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody