Rabbit Anti-CA9 Recombinant Antibody (clone 4F12) (CAT#: ZG-0578J)

This product is a rabbit antibody that recognizes CA9.


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IHC

Figure 1 Rabbit Anti-CA9 Antibody (ZG-0578J) in IHC

Figure 1 Rabbit Anti-CA9 Antibody (ZG-0578J) in IHC

IHC image of ZG-0578J diluted at 1:118 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC

Figure 2 Rabbit Anti-CA9 Antibody (ZG-0578J) in IHC

Figure 2 Rabbit Anti-CA9 Antibody (ZG-0578J) in IHC

IHC image of ZG-0578J diluted at 1:118 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.


Specifications

  • Immunogen
  • A synthesized peptide derived from human CA9
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human CA9
  • Species Reactivity
  • Human
  • Clone
  • 4F12
  • Applications
  • ELISA, IHC
  • Conjugate
  • Uconjugated
  • Related Disease
  • Cardiovascular

Product Property

  • Purification
  • Affinity purified
  • Format
  • Liquid
  • Concentration
  • See the COA
  • Buffer
  • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
    IHC:1:50-1:200

Target

  • Alternative Names
  • MN; CAIX; carbonic anhydrase 9; CA-IX; P54/58N; RCC-associated antigen G250; RCC-associated protein G250; carbonate dehydratase IX; carbonic anhydrase IX; carbonic dehydratase; membrane antigen MN; pMW1; renal cell carcinoma-associated antigen G250

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "CA9"

Recombinant Antibody

Chimeric Antibody

CAT Product Name Application Type
TAB-707 Anti-CAIX/CA9 Recombinant Antibody (Girentuximab) WB, FuncS, IF, Neut, ELISA, FC, IP IgG1 - kappa
TAB-H43 Anti-Human CA9 Recombinant Antibody (Iodine (124I) Girentuximab) ELISA, IP, IF, Inhib IgG1 - kappa

Human Antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0427 Hi-Affi™ Rabbit Anti-CA9 Recombinant Antibody (clone DS427AB) IHC-P, WB Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-H43 Afuco™ Anti-CA9 ADCC Recombinant Antibody (Girentuximab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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