Rabbit Anti-CCND3 Recombinant Antibody (VS3-CJ865) (CAT#: VS3-CJ865)
This product is a rabbit antibody that recognizes human, and mouse CCND3.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42871/1432_F9_3_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42871/1432_F9_3_selected.jpg
Figure 2 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_B_9_5.jpg
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_B_9_5.jpg
Figure 3 Cerebellum
(Cells in granular layer Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in molecular layer Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_B_8_8.jpg
(Cells in granular layer Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in molecular layer Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_B_8_8.jpg
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_9_3.jpg
(Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_9_3.jpg
Figure 5 Testis
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_5_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_5_6.jpg
Figure 6 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_7_8.jpg
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_7_8.jpg
Figure 7 RNA cell line category: Cell line enhanced (MOLT-4, REH)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112576-CCND3
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112576-CCND3
Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse CCND3
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry.
Target
- Alternative Names
- Cyclin D3
- Gene ID
- 896
- UniProt ID
- P30281
- Sequence Similarities
- Belongs to the cyclin family. Cyclin D subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation at Thr-283 by MAP kinases is required for ubiquitination and degradation by the DCX(AMBRA1) complex.
Ubiquitinated by the DCX(AMBRA1) complex during the transition from G1 to S cell phase, leading to its degradation: ubiquitination is dependent on Thr-283 phosphorylation (PubMed:33854235, PubMed:33854239).
The DCX(AMBRA1) complex represents the major regulator of CCND3 stability during the G1/S transition (PubMed:33854235, PubMed:33854239).
Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation (PubMed:22020328).
- Protein Refseq
- NP_001129489.1; NP_001129597.1; NP_001129598.1
- Function
- Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition (PubMed:8114739).
Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase (PubMed:8114739).
Hypophosphorylates RB1 in early G1 phase (PubMed:8114739).
Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed:8114739).
Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (PubMed:16782892).
Shows transcriptional coactivator activity with ATF5 independently of CDK4 (PubMed:15358120).
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
Hedgehog Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CCND3"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0111CT | Mouse Anti-CCND3 Recombinant Antibody (clone 7I5) | ELISA, WB | Mouse IgG1 |
| MRO-0414-CN | Rabbit Anti-CCND3 Recombinant Antibody (clone CBACN-165) | WB, IF, FC | Rabbit IgG |
| ZG-0448F | Mouse Anti-CCND3 Recombinant Antibody (ZG-0448F) | WB, ELISA | Mouse IgG |
| ZG-0793F | Mouse Anti-CCND3 Recombinant Antibody (clone DCS2.2) | WB, IP, IHC | Mouse IgG1 |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-265CQ | Mouse Anti-CCND3 Recombinant Antibody (clone CBL926) | FC, IF, IHC, IP, WB, Neut | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0516 | Hi-Affi™ Rabbit Anti-CCND3 Recombinant Antibody (clone DS516AB) | FC, IHC-P, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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