Mouse Anti-CD14 Recombinant Antibody (clone 1A7C5) (CAT#: ZG-0243U)

Figure 1 Mouse Anti-CD14 Antibody (ZG-0243U) in WB

Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate All lanes: CD14 antibody at 1: 1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 55 kDa

Figure 2 Mouse Anti-CD14 Antibody (ZG-0243U) in IHC

IHC image of ZG-0243U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 3 Mouse Anti-CD14 Antibody (ZG-0243U) in IF

Immunofluorescence staining of HepG2 cells with ZG-0243U at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).