Mouse Anti-CD274 Recombinant Antibody (clone 9B5F4) (CAT#: ZG-0238U)

Figure 1 Mouse Anti-CD274 Antibody (ZG-0238U) in WB

Positive WB detected in: A549 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate All lanes: PD-L1 antibody at 1: 2500
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 34, 21 kDa
Observed band size: 55 kDa

Figure 2 Mouse Anti-CD274 Antibody (ZG-0238U) in IHC

IHC image of ZG-0238U diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 3 Mouse Anti-CD274 Antibody (ZG-0238U) in IF

Immunofluorescence staining of A549 cells with ZG-0238U at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).

Figure 4 Mouse Anti-CD274 Antibody (ZG-0238U) in FC

Overlay histogram showing 293 cells stained with ZG-0238U (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.