Mouse Anti-CD40 Recombinant Antibody (clone Lob 7/4) (CAT#: PABC-256)

Figure 1 Western blotting

C-terminally His-tagged CD40 proteins consisting of 4, 3, or 2 CRD domains (4 = full-length; 3 = CRD1 deleted; 2 = CRD1 and CRD2 deleted) were analyzed by western blotting with the mAbs indicated above each panel used for detection. A composite image from multiple blots with different antibodies is shown.

Yu, X., Chan, H. C., Orr, C. M., Dadas, O., Booth, S. G., Dahal, L. N., ... & Duriez, P. (2018). Complex interplay between epitope specificity and isotype dictates the biological activity of anti-human CD40 antibodies. Cancer cell, 33(4), 664-675.

Figure 2 Flow cytometry

HCD0Tg mouse B cells were incubated with FITC-labeled Lob 7/4 or Lob 7/6, as indicated, in the absence or presence of a 10-fold excess of competitor (comp) mAbs indicated above each plot. Cell labeling was assessed by flow cytometry; filled histogram, unlabeled cells; light gray, FITC-labeled mAbs alone; black line, FITC-labeled mAbs + competitor.

Yu, X., Chan, H. C., Orr, C. M., Dadas, O., Booth, S. G., Dahal, L. N., ... & Duriez, P. (2018). Complex interplay between epitope specificity and isotype dictates the biological activity of anti-human CD40 antibodies. Cancer cell, 33(4), 664-675.

Figure 3 Surface plasmon resonance (SPR) analysis

Surface plasmon resonance analysis of mAb competition for CD40 binding. The mAbs indicated above each plot were flowed over immobilized hCD40 ECD for 800 s to allow saturation of hCD40 binding sites, followed by the addition of individual competitor mAb for 350 s. Non-competitive mAbs (i.e., mAbs without overlapping epitope) give a response greater than 500 response units (RU).

Yu, X., Chan, H. C., Orr, C. M., Dadas, O., Booth, S. G., Dahal, L. N., ... & Duriez, P. (2018). Complex interplay between epitope specificity and isotype dictates the biological activity of anti-human CD40 antibodies. Cancer cell, 33(4), 664-675.