Rabbit Anti-CHEK1 Recombinant Antibody (clone R02-1D6) (CAT#: VS3-XY3314)
It is a recombinant rabbit antibody that specifically recognizes human CHEK1 (Checkpoint kinase 1), which regulates the transcription of genes that regulate cell-cycle progression through the phosphorylation of histones. It also has cross-reactivity against mouse or rat CHEK1. It has been reported for use in Western Blot, Immunoprecipitation, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/if_selected.jpg
Figure 2 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/804_D1_1_red_green.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/804_D1_1_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/712_D1_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/712_D1_1_red_green.jpg
Figure 2 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000149554-CHEK1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000149554-CHEK1
Figure 4 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149554-CHEK1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149554-CHEK1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CHEK1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-1D6
- Applications
- WB, ICC, IF, IP
- Related Disease
- Oocyte/Zygote/Embryo Maturation Arrest 21; Ataxia-Telangiectasia
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- CHK1; OZEMA21
- Gene ID
- 1111
- UniProt ID
- O14757
- Long Name
- Checkpoint kinase 1
- Cellular Localization
- Chromosome, Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys132, Lys180, Lys244, Lys313, and Lys436 (NX_O14757 [NX_O14757-1])
Modification sites at PhosphoSitePlus (O14757)
Glycosylation from GlyGen (O14757) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011540862.1; XP_024304105.1
- Function
- May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T].
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CHEK1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0005CT | Recombinant Mouse anti-Human CHEK1 Monoclonal antibody (7G6) | WB | |
| MRO-0359-CN | Rabbit Anti-CHEK1 Recombinant Antibody (clone CBACN-146) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0419F | Mouse Anti-CHEK1 Recombinant Antibody (ZG-0419F) | WB, ELISA | Mouse IgG |
| VS3-CJ127 | Rabbit Anti-CHEK1 Recombinant Antibody (VS3-CJ127) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS3-CJ128 | Rabbit Anti-CHEK1 Recombinant Antibody (VS3-CJ128) | WB, IHC | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0688 | Hi-Affi™ Rabbit Anti-CHEK1 Recombinant Antibody (clone DS688AB) | IP, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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