Human Anti-CHIKV E2 Recombinant Antibody (clone ch-CHK-152 N297Q) (CAT#: HPAB-S0185-YC)
A chimeric mouse-human ch-CHK-152 N297Q, an aglycosyl variant, was derived from murine antibody CHK-152, which lacks the ability to engage C1q or Fc-γ receptors; this mutation does not affect the ability to bind the neonatal Fc receptor (FcRn) or half-life of antibody in mouse serum. The affinity of ch-CHK-152 N297Q binding to purified pE2-E1 was measured by surface plasmon resonance (SPR) and compared to the parent murine MAb. Notably, ch-CHK-152 N297Q and the murine CHK-152 all had similar affinity (KD of 3 to 4 nM) and neutralizing activity in cell culture (Fig. 4B). As expected, ch-CHK-152 N297Q failed to bind efficiently to soluble Fc-γ receptors or C1q.
Specific Inquiry
Figure 1 The effector functions of CHK-152 contribute to protection in vivo.
ch-CHK-152, ch-CHK-152 N297Q, and the murine CHK-152 all had similar affinity and neutralizing activity in cell culture.
Pal, P., Dowd, K. A., Brien, J. D., Edeling, M. A., Gorlatov, S., Johnson, S., ... & Diamond, M. S. (2013). Development of a highly protective combination monoclonal antibody therapy against Chikungunya virus. PLoS pathogens, 9(4), e1003312.
Specifications
- Immunogen
- Adult C57BL/6 mice deficient for interferon regulatory factor 7 (Irf7 −/−) were infected with the La Reunion 2006 OPY-1 strain of CHIKV (CHIKV-LR). These mice were boosted with CHIK virus-like particles, soluble recombinant CHIKV E2 protein, or live CHIKV-LR.
- Host Species
- Human
- Derivation
- Chimeric (mouse/human)
- Type
- Human IgG
- Specificity
- CHIKV E2
- Species Reactivity
- CHIKV
- Clone
- ch-CHK-152 N297Q
- Applications
- Neut, FRNT, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Chikungunya virus E2 protein
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-430 | Recombinant Human Anti-CHIKV E2 Antibody (CHK265) | IB, ELISA, BL | IgG |
| PABL-431 | Recombinant Mouse Anti-CHIKV E2 Antibody (CHK9) | Neut, BL | IgG |
| MOB-152CQ | Mouse Anti-CHIKV E2 Antibody (CHK265) | Neut, ELISA, IF, FC | Mouse IgG1, λ |
| HPAB-S0184-YC | Human Anti-CHIKV E2 Recombinant Antibody (clone ch-CHK-152) | Neut, FRNT, FuncS | Human IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-427 | Recombinant Human Anti-CHIKV E2 Antibody Fab Fragment (CHK265) | Neut | Fab |
| PFBL-428 | Recombinant Mouse Anti-CHIKV E2 Antibody Fab Fragment (CHK9) | Neut, BL | Fab |
| HPAB-S0184-YC-F(E) | Human Anti-CHIKV E2 Recombinant Antibody (clone ch-CHK-152); Fab Fragment | Neut, FRNT, FuncS | Human Fab |
| HPAB-S0185-YC-F(E) | Human Anti-CHIKV E2 Recombinant Antibody (clone ch-CHK-152 N297Q); Fab Fragment | Neut, FRNT, FuncS | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-427 | Recombinant Human Anti-CHIKV E2 Antibody scFv Fragment (CHK265) | Neut | scFv |
| PSBL-428 | Recombinant Mouse Anti-CHIKV E2 Antibody scFv Fragment (CHK9) | Neut, BL | scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1158LC | Anti-CHIKV E2 Recombinant Antibody (3C3) | ELISA, WB, IF, FC | Mouse Antibody |
| MRO-1159LC | Anti-CHIKV E2 Recombinant Antibody (3E4) | ELISA, WB, IF, FC | Mouse Antibody |
| MRO-1158LC-F(E) | Anti-CHIKV E2 Recombinant Antibody Fab Fragment (3C3) | ELISA, WB, IF | Mouse Antibody |
| MRO-1159LC-F(E) | Anti-CHIKV E2 Recombinant Antibody Fab Fragment (3E4) | ELISA, WB, IF | Mouse Antibody |
| MRO-1160LC-F(E) | Anti-CHIKV E2 Recombinant Antibody Fab Fragment (8A4) | ELISA, WB, IF | Mouse Antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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