Provided is an anti-interleukin-8 (IL-8) monoclonal antibody 6G4.2.5. The antibody may be used in diagnostic applications and in the treatment studies of inflammatory disorders.
Figure 1 The anti-IL-8 mAb pair of 5A.12.14 and 6G4.2.5 allows for detection of IL-8 by ELISA.
It indicates that the successful detection of IL-8 using the two different antibodies cannot be attributed to the IL-8 existing in a dimeric state and demonstrates that
6G4.2.5 and A5.12.14 bind to separate epitopes on the IL-8 molecule.
Deforge, L. E., Lowman, H. B., Leong, S. R., Chuntharapai, A., Kim, K. J., & Hébert, C. A. (2000). A neutralizing monoclonal antibody specific for the dimer interface region of IL-8. Cytokine, 12(11), 1620-1629.
Figure 2 A5.12.14 and 6G4.2.5 dose-dependently inhibit IL-8-stimulated release of β-glucuronidase from human neutrophils.
IL-8+A5.12.14, -●-; IL-8+6G4.2.5, -□-; IL-8 alone,◆ ; A5.12.14 alone,△ ; 6G4.2.5 alone, ▼. ID50 (nM): 6G4.2.5, 10.16; A5.12.14, 4.70. Keeping the IL-8 concentration constant at 10 nM, 6G4.2.5 or A5.12.14 was added over a concentration range of 0.65-50 nM and caused a dose-dependent inhibition of β- glucuronidase release.
Deforge, L. E., Lowman, H. B., Leong, S. R., Chuntharapai, A., Kim, K. J., & Hébert, C. A. (2000). A neutralizing monoclonal antibody specific for the dimer interface region of IL-8. Cytokine, 12(11), 1620-1629.
Figure 3 A5.12.14 and 6G4.2.5 detection of wild-type IL-8 and the four monomer variants by ELISA.
A blank (●) was run on each plate in which an equivalent volume of assay diluent was added in place of the mAbs. A: while A5.12.14 bound nearly identically to wild-type IL-8 (-■-) and variant II (--◆--), the binding curve for variant I (- ◯ -) was shifted to the right, and variants III (-▼-) and IV (--△--) were not detected. B: 6G4.2.5 was capable of binding to all of the monomer mutants, although these curves were shifted to the right as compared to wild-type IL-8. The results represent the average of optical density values of duplicate samples. 6G4.2.5 was able to bind to all of the monomer variants in this ELISA format, although the curves were shifted to the right relative to wild-type IL-8.
Deforge, L. E., Lowman, H. B., Leong, S. R., Chuntharapai, A., Kim, K. J., & Hébert, C. A. (2000). A neutralizing monoclonal antibody specific for the dimer interface region of IL-8. Cytokine, 12(11), 1620-1629.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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NAB-738-sdAb | Recombinant Anti-human CXCL8 VHH Single Domain Antibody | IHC, ICC, FC, FA, FUNC | Llama VHH |
CAT | Product Name | Application | Type |
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TAB-201CL | Human Anti-CXCL8 Recombinant Antibody (TAB-201CL) | ELISA | Human IgG2 |
TAB-701LC | Human Anti-CXCL8 Recombinant Antibody (TAB-701LC) | ELISA, FC, FuncS | Human IgG1, κ |
TAB-701LC-S(P) | Human Anti-CXCL8 Recombinant Antibody; scFv Fragment (TAB-701LC-S(P)) | ELISA | Human scFv |
TAB-701LC-F(E) | Human Anti-CXCL8 Recombinant Antibody; Fab Fragment (TAB-701LC-F(E)) | ELISA | Human Fab |
CAT | Product Name | Application | Type |
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Gly-095LC | Recombinant Anti-Human CXCL8 Antibody (Fc glycosylation) | ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-164LC | Recombinant Anti-Human CXCL8 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-644CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone CBL601) | ELISA, Neut | Mouse IgG1 |
NEUT-645CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone CBL602) | WB, ICC, Neut | Mouse IgG1 |
NEUT-646CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone CBL115) | FC, WB, IHC, Neut | Mouse IgG1 |
NEUT-647CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone I8-60) | ELISA, Neut, WB | Mouse IgG1, κ |
NEUT-648CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone I8-S2) | ELISA, Neut, WB | Mouse IgG2b, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-660CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone B-K8) | Block, ICFC | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4128 | Hi-Affi™ Rabbit Anti-CXCL8 Recombinant Antibody (clone SI199DS) | ELISA | Rabbit IgG |
MOR-4624 | Hi-Affi™ Rabbit Anti-CXCL8 Recombinant Antibody (clone TH137DS) | WB | Rabbit IgG |
MOR-4625 | Hi-Affi™ Rabbit Anti-CXCL8 Recombinant Antibody (clone TH138DS) | WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-1973-CN | Rabbit Anti-CXCL8 Polyclonal Antibody (MRO-1973-CN) | ELISA, IF, IHC | Rabbit IgG |
MRO-1974-CN | Rabbit Anti-CXCL8 Polyclonal Antibody (MRO-1974-CN) | WB, IF, FC | Rabbit IgG |
HPAB-1501-FY | Mouse Anti-CXCL8 Recombinant Antibody (clone 5D2.2D10) | ELISA, WB | Mouse IgG1 |
HPAB-1502-FY | Mouse Anti-CXCL8 Recombinant Antibody (clone 1A1.4C6) | ELISA, WB | Mouse IgG1 |
HPAB-1503-FY | Mouse Anti-CXCL8 Recombinant Antibody (clone 1L351.17) | ELISA, WB | Mouse IgG2b |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-N0059-YC-S(P) | Human Anti-CXCL8 Recombinant Antibody; scFv Fragment (HPAB-N0059-YC-S(P)) | FuncS | Human scFv |
HPAB-1311WJ-S(P) | Human Anti-CXCL8 Recombinant Antibody (clone M2-11); scFv Fragment | ELISA | Human scFv |
HPAB-S0085-YC-S(P) | Human Anti-CXCL8 Recombinant Antibody (clone ch6G4.2.5); scFv Fragment | ELISA, Inhib | Mouse scFv |
HPAB-S0086-YC-S(P) | Human Anti-CXCL8 Recombinant Antibody (clone h6G4V11); scFv Fragment | ELISA, Inhib | Humanized scFv |
HPAB-S0087-YC-S(P) | Human Anti-CXCL8 Recombinant Antibody (clone h6G4V11N35A); scFv Fragment | ELISA, Inhib, FuncS | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-1101-FY-S(P) | Mouse Anti-CXCL8 Recombinant Antibody (clone 656.35); Fab Fragment | ELISA, IA, Block | Mouse Fab |
HPAB-1102-FY-S(P) | Mouse Anti-CXCL8 Recombinant Antibody (clone 197.2); Fab Fragment | ELISA, IA, Block | Mouse Fab |
HPAB-1103-FY-S(P) | Mouse Anti-CXCL8 Recombinant Antibody (clone 81.1); Fab Fragment | ELISA, IA, Block | Mouse Fab |
HPAB-1104-FY-S(P) | Mouse Anti-CXCL8 Recombinant Antibody; scFv Fragment (HPAB-1104-FY-S(P)) | ELISA | Mouse scFv |
HPAB-0664-YJ-F(E) | Mouse Anti-CXCL8 Recombinant Antibody (clone 5.12.14); Fab Fragment | ELISA, WB, Inhib, Antagonist | Mouse Fab |
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