Recombinant Mouse Anti-DERP1 Antibody (W108) (CAT#: HPAB-0068-WJ)

Figure 1 The competitive binding analysis of monoclonal antibody W108 using patient IgE to Der p allergen.

a. Binding of Der psensitive sera (white bar) and pre-incubated with mAb W108 (black bar) detected using HRP-mouse anti-human IgE Ab, respectively.

Dai, Y. C., Chuang, W. J., Chua, K. Y., Shieh, C. C., & Wang, J. Y. (2011). Epitope mapping and structural analysis of the anti-Der p 1 monoclonal antibody: insight into therapeutic potential. Journal of molecular medicine, 89(7), 701-712.

Figure 2 The competitive binding analysis of monoclonal antibody W108 using patient IgE to Der p allergen.

b. The mAb W108 had the highest blocking activity (inhibited more than 70% of the allergen-specific IgE binding in the pooled sera [empty circle] of nine Der p-sensitive allergic asthmatic children).

Dai, Y. C., Chuang, W. J., Chua, K. Y., Shieh, C. C., & Wang, J. Y. (2011). Epitope mapping and structural analysis of the anti-Der p 1 monoclonal antibody: insight into therapeutic potential. Journal of molecular medicine, 89(7), 701-712.

Figure 3 The competitive binding analysis of monoclonal antibody W108 using patient IgE to Der p allergen.

c. Immunoblot analysis of mAb W108 and patient sera to Der p allergen.

Dai, Y. C., Chuang, W. J., Chua, K. Y., Shieh, C. C., & Wang, J. Y. (2011). Epitope mapping and structural analysis of the anti-Der p 1 monoclonal antibody: insight into therapeutic potential. Journal of molecular medicine, 89(7), 701-712.

Figure 4 The competitive binding analysis of monoclonal antibody W108 using patient IgE to Der p allergen.

d. The cysteine protease activity of Der p 1 (100 μg/ml) (using azocasein as a substrate) is inhibited by 100 ng/ml (empty diamond) and 40 ng/ml (filled diamond) of mAbW108. Der p 1 (100 μg/ml) alone (filled circle); Der p 1 (100 μg/ml) incubated with IgG (100 ng/ml) (empty circl).

Dai, Y. C., Chuang, W. J., Chua, K. Y., Shieh, C. C., & Wang, J. Y. (2011). Epitope mapping and structural analysis of the anti-Der p 1 monoclonal antibody: insight into therapeutic potential. Journal of molecular medicine, 89(7), 701-712.

Figure 5 The effect of mAb W108 on Der p-sensitized and allergenchallenged mice.

The data show that treatment with mAb W108 (1 μg/kg) i.p. during Der p sensitization and 6 h before allergen challenge. Nontreated (black bar), mAb W108 treated (white bar), and IgG treated (striped bar). a Lung histology (H & E stain ×100).

Dai, Y. C., Chuang, W. J., Chua, K. Y., Shieh, C. C., & Wang, J. Y. (2011). Epitope mapping and structural analysis of the anti-Der p 1 monoclonal antibody: insight into therapeutic potential. Journal of molecular medicine, 89(7), 701-712.