Human Anti-DSG3 Recombinant Antibody (clone PVE4-8) (CAT#: FAMAB-0515WJ)
This product is a human monoclonal antibody that is specific for Human DSG3. This antibody can be used in a variety of applications, such as ELISA, WB, IF.
Specific Inquiry
IF
Figure 1 Indirect IF staining of human skin with scFv mAbs demonstrates cell surface binding typical of PV.
Negative control is an scFv mAb against an irrelevant Ag. Positive control is a previously characterized anti-Dsg3 scFv mAb, (D31)2/2918. Scale bar, 20 μM.
Cho, M. J., Lo, A. S., Mao, X., Nagler, A. R., Ellebrecht, C. T., Mukherjee, E. M., ... & Payne, A. S. (2014). Shared VH1-46 gene usage by pemphigus vulgaris autoantibodies indicates common humoral immune responses among patients. Nature communications, 5(1), 1-11.
Figure 2 VH1-46 mAbs, but not VH5a mAb, induce suprabasal skin blisters in human skin organ culture. Scale bar, 100 μM.
Cho, M. J., Lo, A. S., Mao, X., Nagler, A. R., Ellebrecht, C. T., Mukherjee, E. M., ... & Payne, A. S. (2014). Shared VH1-46 gene usage by pemphigus vulgaris autoantibodies indicates common humoral immune responses among patients. Nature communications, 5(1), 1-11.
ELISA
Figure 3 GL LC/HC recombinant Abs were generated for those mAbs that were insoluble in their fully GL state.
(a) F706 germline-reverted (GL) and (b) F779GL Abs do not bind Dsg3. (c) 3.2 GL Ab binds Dsg3 by ELISA with reduced affinity. (d) PVE4-8 GL and (e) 4.2 GL Abs retain binding to Dsg3 by ELISA. Black/grey hatched lines indicate GL1/2 Abs, respectively. (f-j) Germline-reverted non-VH1-46 mAbs do not demonstrate binding to Dsg3 by ELISA.
Cho, M. J., Lo, A. S., Mao, X., Nagler, A. R., Ellebrecht, C. T., Mukherjee, E. M., ... & Payne, A. S. (2014). Shared VH1-46 gene usage by pemphigus vulgaris autoantibodies indicates common humoral immune responses among patients. Nature communications, 5(1), 1-11.
IF
Figure 4 Indirect IF (IIF) staining of human skin confirms Dsg3 autoreactivity of PVE4-8 GL and 4.2 GL Abs in native skin tissue.
IIF binding of 3.2 GL was not detectable, presumably due to low affinity. Scale bar, 20 μM. Error bars indicate s.e.m. Data are representative of three to five independent experiments.
Cho, M. J., Lo, A. S., Mao, X., Nagler, A. R., Ellebrecht, C. T., Mukherjee, E. M., ... & Payne, A. S. (2014). Shared VH1-46 gene usage by pemphigus vulgaris autoantibodies indicates common humoral immune responses among patients. Nature communications, 5(1), 1-11.
Figure 5 PVE4-8GL retains binding to Dsg3, which depends on three acidic residues in the heavy chain CDR3 (PVE4-8 GL HC D>N).
Scale bar, 20 μM.
Cho, M. J., Lo, A. S., Mao, X., Nagler, A. R., Ellebrecht, C. T., Mukherjee, E. M., ... & Payne, A. S. (2014). Shared VH1-46 gene usage by pemphigus vulgaris autoantibodies indicates common humoral immune responses among patients. Nature communications, 5(1), 1-11.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human DSG3
- Species Reactivity
- Human
- Clone
- PVE4-8
- Applications
- ELISA, WB, IF
- Related Disease
- Pemphigus vulgaris
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "DSG3"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-172 | Human Anti-DSG3 Recombinant Antibody (clone PVA3) | ELISA | Human IgG |
| FAMAB-1111CQ | Human Anti-DSG3 Recombinant Antibody (clone PVA224) | IF, ELISA, IHC | Human IgG1 |
| FAMAB-1113CQ | Mouse Anti-DSG3 Recombinant Antibody (clone PVB28) | IF, ELISA, IHC | Mouse IgG1 |
| FAMAB-1114CQ | Human Anti-DSG3 Recombinant Antibody (clone PVA232) | ELISA | Human IgG1 |
| FAMAB-1115CQ | Human Anti-DSG3 Recombinant Antibody (clone PVB16) | IF, ELISA, IHC | Human IgG1 |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-258MZ | Anti-Human DSG3 Recombinant Antibody (DF151) | IHC, FC | |
| TAB-259MZ | Anti-Human DSG3 Recombinant Antibody (DF364) | IHC, FC | |
| TAB-260MZ | Anti-Human DSG3 Recombinant Antibody (DF366) | IHC, FC | |
| TAB-258MZ-S(P) | Anti-Human DSG3 Recombinant Antibody scFv Fragment (DF151) | IHC, FC | |
| TAB-259MZ-S(P) | Anti-Human DSG3 Recombinant Antibody scFv Fragment (DF364) | IHC, FC |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1055 | Hi-Affi™ Rabbit Anti-DSG3 Recombinant Antibody (clone DS1055AB) | WB | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0203-CN-S(P) | Human Anti-DSG3 Recombinant Antibody (clone (D3)1b/3a); scFv Fragment | ELISA, IF, IB | Human scFv |
| FAMAB-0204-CN-S(P) | Human Anti-DSG3 Recombinant Antibody (clone (D3);3c/9); scFv Fragment | ELISA, IF, IB | Human scFv |
| FAMAB-0205-CN-S(P) | Human Anti-DSG3 Recombinant Antibody (clone (D3)3a/9); scFv Fragment | ELISA, IF, IB | Human scFv |
| FAMAB-0152JF-S(P) | Human Anti-DSG3 Recombinant Antibody (clone PVMAB786); scFv Fragment | IF, WB, ELISA, FuncS | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0203-CN-F(E) | Human Anti-DSG3 Recombinant Antibody (clone (D3)1b/3a); Fab Fragment | ELISA, IF, IB | Human Fab |
| FAMAB-0204-CN-F(E) | Human Anti-DSG3 Recombinant Antibody (clone (D3)3c/9); Fab Fragment | ELISA, IF, IB | Human Fab |
| FAMAB-0205-CN-F(E) | Human Anti-DSG3 Recombinant Antibody (clone (D3)3a/9); Fab Fragment | ELISA, IF, IB | Human Fab |
| FAMAB-0152JF-F(E) | Human Anti-DSG3 Recombinant Antibody (clone PVMAB786); Fab Fragment | IF, WB, ELISA, FuncS | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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