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  • Rabbit Anti-ELAVL1 Recombinant Antibody (clone R04-3A6)

Rabbit Anti-ELAVL1 Recombinant Antibody (clone R04-3A6) (CAT#: VS3-XY3343)

This antibody is a recombinant rabbit antibody binding with ELAVL1 (ELAV like RNA binding protein 1), which involves in embryonic stem cell (ESC) differentiation. It reacts with human, mouse and rat ELAVL1. It has been detected using Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.


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Subcellular Location
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human parathyroid gland
Figure 2 IHC staining of human parathyroid gland
(Immunohistochemical staining of human parathyroid gland shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/ihc_selected.jpg
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/13674_B_7_5.jpg
Figure 4 Colon
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/13674_A_7_3.jpg
Figure 5 Kidney
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/13674_A_8_5.jpg
Figure 6 Testis
Figure 6 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5256/13674_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000066044-ELAVL1

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat ELAVL1
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R04-3A6
  • Applications
  • WB, IHC-P, ICC, IF, IP, FC
  • Related Disease
  • Colorectal Cancer; Ureteral Obstruction

Product Property

  • Clonality
  • Monoclonal
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl and 50% glycerol
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • WB: 1/500-1/1000
    IHC: 1/50-1/100
    IF: 1/50-1/200
    IP: 1/20
    FC: 1/50-1/100

Target

  • Alternative Names
  • HUR; Hua; MelG; ELAV1
  • Long Name
  • ELAV like RNA binding protein 1
  • Cellular Localization
  • Nucleus, Cytoplasm, Stress granule
  • Post Translation Modifications
  • Phosphorylated by MAPKAPK2 (PubMed:14517288).
    Phosphorylated by PRKCD (PubMed:18285462).
    (Q15717-ELAV1_HUMAN)
    Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge. (Q15717-ELAV1_HUMAN)
    Ubiquitination at Lys50, Lys55, Lys72, Lys104, Lys120, Lys191, Lys313, and Lys320 (NX_Q15717 [NX_Q15717-1])
    Modification sites at PhosphoSitePlus (Q15717)
    Glycosylation from GlyGen (Q15717) 1 site, 1 O-linked glycan (1 site)
  • Function
  • RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed:14517288, 18285462, 31358969).
    Involved in embryonic stem cell (ESC) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation.

Recommended Products

Isotype Control

Recommended Dilution Buffer

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "ELAVL1"

Recombinant Antibody

CAT Product Name Application Type
MOB-1825MZ Recombinant Mouse Anti-Human ELAVL1 Antibody WB Mouse antibody
MRO-0803-CN Rabbit Anti-ELAVL1 Recombinant Antibody (clone JM96-33) WB, IHC, IP, FC Rabbit IgG
VS3-WK995 Mouse Anti-ELAVL1 Recombinant Antibody (VS3-WK995) WB, ICC Mouse IgG

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC2088 PE-B*07:02/Human ELAVL1 (NPNQNKNVAL) MHC Tetramer FCM
MHC-LC2089 APC-B*07:02/Human ELAVL1 (NPNQNKNVAL) MHC Tetramer FCM

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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