Mouse anti-ENO1 antibody (clone 8H9G12) displayed high specificity against the ENO1 protein of Human, Mouse, Rat and Rabbit.
Figure 1 Mouse Anti-ENO1 Antibody (ZG-0250U) in WB
Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate. All lanes ENO1 antibody at 1: 10000
Secondary
Goat polyclonal to mouse IgG at 1/10000 dilution
Predicted band size: 47 KDa
Observed band size: 47 KDa
Exposure time: 10s
Figure 2 Mouse Anti-ENO1 Antibody (ZG-0250U) in IHC
IHC image of ZG-0250U diluted at 1:500 and staining in paraffin-embedded human colon cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Figure 3 Mouse Anti-ENO1 Antibody (ZG-0250U) in IF
Immunofluorescence staining of HepG2 cells with ZG-0250U at 1:130, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).
Figure 4 Mouse Anti-ENO1 Antibody (ZG-0250U) in FC
Overlay histogram showing Hela cells stained with ZG-0250U (red line) at 1:260. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-350MZ | Anti-Human ENO1 Recombinant Antibody (A3) | WB, FC | Human antibody |
TAB-350MZ-S(P) | Anti-Human ENO1 Recombinant Antibody scFv Fragment (A3) | WB, FC | Human antibody |
TAB-350MZ-F(E) | Anti-Human ENO1 Recombinant Antibody Fab Fragment (A3) | WB, FC | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-0661CT | Recombinant Mouse anti-Human ENO1 Monoclonal antibody (3H3BH22CG9) | FC, ICC/IF, IP, ELISA | |
FAMAB-0235-CN | Chicken Anti-ENO1 Recombinant Antibody (clone EnL2) | ELISA, WB, IF, FC | Chicken IgY |
FAMAB-0236-CN | Chicken Anti-ENO1 Recombinant Antibody (clone EnL5) | ELISA, WB, IF, FC | Chicken IgY |
MRO-0535-CN | Rabbit Anti-ENO1 Recombinant Antibody (clone JM63-53) | WB, IP, FC, IF | Rabbit IgG |
HPAB-J0072-YC | Human Anti-ENO1 Recombinant Antibody (clone B3) | ELISA | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0190MZ | Chicken Anti-ENO1 Polyclonal IgY | WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-LC842 | PE-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer | FCM | |
MHC-LC843 | APC-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer | FCM | |
MHC-LC844 | BV421-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer | FCM |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1151 | Hi-Affi™ Rabbit Anti-ENO1 Recombinant Antibody (clone DS1151AB) | WB, ICC, IF, IP | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0054-CN-F(E) | Mouse Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0054-CN-F(E)) | FC, ELISA | Mouse Fab |
HPAB-0055-CN-F(E) | Human Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0055-CN-F(E)) | FC, ELISA | Humanized Fab |
HPAB-J0072-YC-F(E) | Human Anti-ENO1 Recombinant Antibody (clone B3); Fab Fragment | ELISA | Human Fab |
HPAB-J0073-YC-F(E) | Human Anti-ENO1 Recombinant Antibody (clone C4); Fab Fragment | ELISA | Human Fab |
HPAB-J0074-YC-F(E) | Human Anti-ENO1 Recombinant Antibody (clone B8); Fab Fragment | ELISA | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0235-CN-S(P) | Chicken Anti-ENO1 Recombinant Antibody (clone EnL2); scFv Fragment | ELISA, WB, IF, FC | Chicken scFv |
FAMAB-0236-CN-S(P) | Chicken Anti-ENO1 Recombinant Antibody (clone EnL5); scFv Fragment | ELISA, WB, IF, FC | Chicken scFv |
HPAB-J0072-YC-S(P) | Human Anti-ENO1 Recombinant Antibody (clone B3); scFv Fragment | ELISA | Human scFv |
HPAB-J0073-YC-S(P) | Human Anti-ENO1 Recombinant Antibody (clone C4); scFv Fragment | ELISA | Human scFv |
HPAB-J0074-YC-S(P) | Human Anti-ENO1 Recombinant Antibody (clone B8); scFv Fragment | ELISA | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-YF036 | DRB1*04:01/Human cit alpha-enolase (IFDS[cit]GNPTVEVDLF) MHC Monomer | MHC Multimer |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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