This product is a recombinant antibody that recognizes EPOR. The monoclonal antibody Ab12 reacts with the epitope of EPOR.
Figure 1 Shows a graph showing equilibrium binding of soluble EpoR with various Ab12 scFv constructs expressed on the surface of yeast cells.
Figure 2 Represents an off-rate analysis of Ab12 41/40 scFv.
Figure 3 Shows a graph of EC50 and Emax values of Ab12, AB12.6 and Ab12.6-related antibodies.
EC50 values represent the concentration of antibody or Epo at which 50% maximal cell proliferation is achieved (50% of the slope of a sigmoidal curve). The Emax values represent the maximum number of cells which this EC50 produces (as measured by absorbance).
Figure 4 Shows a graph showing the formation of CFU-E (colony forming unit-erythroid) from human bone marrow in response to treatment with Epogen, Ab12, Aranesp™, Ab12.6 and isotype control.
Figure 5 Shows a graph showing change in hematocrit in mEpoR-/-, hEpoR+transgenic mice over 28 days after administration of a single dose of Ab12.6 on day 0 versus two doses of Aranesp™ administered on day 0 and day 14.
Compared to Ab12, Ab12.6 had improved potency in elevating and maintaining hematocrit levels over a 28 day time period. Ab12.6 at 0.8 mg/kg also caused a faster rate of rise of hematocrit measured on day 7 than a single dose Aranesp™ administered at 3 μg/kg.
Figure 6 Shows the competition of Ab12 with 125I-labeled EPO for binding to Chinese Hamster Ovary cells expressing recombinant EPO receptor.
Figure 7 Shows the results of an EPO dependent human cell proliferation assay using Ab12 and Ab198.
Figure 8 Shows that Ab12 remains active in inducing the proliferation of F36E cells after storage at 4° C. for up to 20 days.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
PABW-040 | Human Anti-EPOR Recombinant Antibody (clone ABT-007) | WB, FuncS | Human IgG |
MOB-0682CT | Recombinant Mouse anti-Human EPOR Monoclonal antibody (EML077) | WB | |
HPAB-0490-CN | Mouse Anti-EPOR Recombinant Antibody (clone 21.3.1) | ELISA, WB, ICC, IHC, IP | Mouse IgG |
HPAB-0491-CN | Mouse Anti-EPOR Recombinant Antibody (clone 19.1.2) | ELISA, WB, ICC, IHC, IP | Mouse IgG |
HPAB-1692-FY | Recombinant Human Anti-EPOR Antibody (Ab12) | Activ, FC, ELISA | Human IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-007WM | Anti-Human EPOR Recombinant Antibody (AB-ABT2-XG2-012) | ELISA | Human Antibody |
TAB-007WM-S(P) | Anti-Human EPOR Recombinant Antibody scFv Fragment (AB-ABT2-XG2-012) | ELISA | Human Antibody |
TAB-007WM-F(E) | Anti-Human EPOR Recombinant Antibody Fab Fragment (AB-ABT2-XG2-012) | ELISA | Human Antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-008WM | Anti-Human EPOR Recombinant Antibody (1E3) | ELISA, WB, FC | |
TAB-008WM-S(P) | Anti-Human EPOR Recombinant Antibody scFv Fragment (1E3) | ELISA, WB, FC | |
TAB-009WM-S(P) | Mouse Anti-EPOR Recombinant Antibody; scFv Fragment (TAB-009WM-S(P)) | WB, FC | Mouse scFv |
TAB-008WM-F(E) | Anti-Human EPOR Recombinant Antibody Fab Fragment (1E3) | ELISA, WB, FC | |
TAB-009WM-F(E) | Mouse Anti-EPOR Recombinant Antibody; Fab Fragment (TAB-009WM-F(E)) | WB, FC | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-736CQ | Mouse Anti-EPOR Recombinant Antibody (clone CBL641) | Neut | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1173 | Hi-Affi™ Rabbit Anti-EPOR Recombinant Antibody (clone DS1173AB) | IP, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0490-CN-F(E) | Mouse Anti-EPOR Recombinant Antibody (clone 21.3.1); Fab Fragment | ELISA, WB, ICC, IHC, IP | Mouse Fab |
HPAB-0491-CN-F(E) | Mouse Anti-EPOR Recombinant Antibody (clone 19.1.2); Fab Fragment | ELISA, WB, ICC, IHC, IP | Mouse Fab |
HPAB-0169CQ-F(E) | Human Anti-EPOR Recombinant Antibody (clone ABT2-SCX-012); Fab Fragment | Agonist, ELISA | Human Fab |
HPAB-0359-YJ-F(E) | Human Anti-EPOR Recombinant Antibody; Fab Fragment (HPAB-0359-YJ-F(E)) | ELISA, FC, Activ, FuncS | Human Fab |
HPAB-0360-YJ-F(E) | Human Anti-EPOR Recombinant Antibody; Fab Fragment (HPAB-0360-YJ-F(E)) | ELISA, FC, Activ, FuncS | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-1692-FY-F(E) | Recombinant Human Anti-EPOR Antibody scFv Fragment (Ab12) | FC, ELISA | Human scFv |
HPAB-1693-FY-F(E) | Human Anti-EPOR Recombinant Antibody (clone Ab12.6); scFv Fragment | FC, ELISA | Human scFv |
HPAB-1694-FY-F(E) | Human Anti-EPOR Recombinant Antibody (clone Ab12.56); scFv Fragment | FC, ELISA | Human scFv |
HPAB-1306WJ-S(P) | Mouse Anti-EPOR Recombinant Antibody (clone 3G9); scFv Fragment | ELISA, WB, Agonist | Mouse scFv |
HPAB-1307WJ-S(P) | Human Anti-EPOR Recombinant Antibody (clone 3G9HZLC); scFv Fragment | ELISA, WB, Agonist | Humanized scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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