Rabbit Anti-FOXO3 Recombinant Antibody (clone 1E1) (CAT#: ZG-0675J)

Figure 1 Rabbit Anti-FOXO3 Antibody (ZG-0675J) in WB

Western Blot
Positive WB detected in Hela whole cell lysate,293 whole cell lysate,A549 whole cell lysate
All lanes Phospho-FOXO3 antibody at 0.96μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 97 KDa
Observed band size: 97 KDa

Figure 2 Rabbit Anti-FOXO3 Antibody (ZG-0675J) in IHC

IHC image of this product diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 3 Rabbit Anti-FOXO3 Antibody (ZG-0675J) in IHC

IHC image of this product diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.