Rabbit Anti-FTO Recombinant Antibody (clone 4G9) (CAT#: ZG-0678J)

Figure 1 Rabbit Anti-FTO Antibody (ZG-0678J) in WB

Western Blot
Positive WB detected in: 293T whole cell lysate, SH-SY5Y whole cell lysate, Raji whole cell lysate, Colo320 whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate
All lanes: FTO antibody at 0.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 59, 15, 7, 13 KDa
Observed band size: 59 KDa

Figure 2 Rabbit Anti-FTO Antibody (ZG-0678J) in IHC

IHC image of this product diluted at 1:70 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 3 Rabbit Anti-FTO Antibody (ZG-0678J) in IHC

IHC image of this product diluted at 1:70 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 4 Rabbit Anti-FTO Antibody (ZG-0678J) in IF

Immunofluorescence staining of Hela cells with this product at 1:23, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).