Human Anti-FZD5 Recombinant Antibody (clone F2.I) (CAT#: PABJ-0145)
This product is a Human antibody that recognizes Human FZD5. F2.I bound FZD1, -2, -4, -5, -7, and -8 on the cell surface, but did not bind FZD3, -6, -9, or -10. F2.I showed cross-reactivity to mouse FZD4 and FZD5.
Specific Inquiry
SDS-PAGE
Figure 1 Human Anti-FZD5 Recombinant Antibody (clone F2.I) (PABJ-0145) in SDS-PAGE
SDS-PAGE analysis of PABJ-0145 in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of PABJ-0145 was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 2 Human Anti-FZD5 Recombinant Antibody (clone F2.I) (PABJ-0145) in HPLC
The purity of PABJ-0145 was greater than 95% as determined by SEC-HPLC.
WB
Figure 3 Human Anti-FZD5 Recombinant Antibody (clone F2.I) (PABJ-0145) in WB
Western blot analysis of PABJ-0145 was performed by loading recombinant human FZD5 protein.
PABJ-0145 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 4 Human Anti-FZD5 Recombinant Antibody (clone F2.I) (PABJ-0145) in ELISA
ELISA analysis of PABJ-0145 was performed by coating with recombinant human FZD5 protein. Then blocked with BSA and incubated with Human Anti-FZD5 Recombinant Antibody (clone F2.I) (PABJ-0145). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human FZD5
- Species Reactivity
- Human
- Clone
- F2.I
- Applications
- ELISA, Inhib, FuncS
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Inhibition, Functional Assay.
Target
- Alternative Names
- HFZ5; C2orf31; frizzled-5; Wnt receptor; frizzled 5, seven transmembrane spanning receptor; frizzled family receptor 5; fz-5; fzE5; seven-transmembrane receptor frizzled-5
- Gene ID
- 7855
- UniProt ID
- Q13467
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0749CT | Recombinant Mouse anti-Human FZD5 Monoclonal antibody (EML2242) | WB | |
| MRO-1876-CN | Rabbit Anti-FZD5 Polyclonal Antibody (MRO-1876-CN) | WB | Rabbit IgG |
| VS3-QX453 | Mouse Anti-FZD5 Recombinant Antibody (clone 2D12) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABJ-0145-S(P) | Human Anti-FZD5 Recombinant Antibody (clone F2.I); scFv Fragment | ELISA, Inhib, FuncS | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABJ-0145-F(E) | Human Anti-FZD5 Recombinant Antibody (clone F2.I); Fab fragment | ELISA, Inhib, FuncS | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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