Human Anti-HIV-1 gp120 Recombinant Antibody (clone 23e) (CAT#: HPAB-1991-FY)

Figure 1 Effects of sCD4 on HMAb binding to HIV-1 gp120.

The gp120 glycoprotein from the YU2 HIV-1 isolate was captured on ELISA plates by the D7324 antibody. The captured gp120 glycoprotein was incubated without sCD4 (open circles) or with 10 mg/ml sCD4 (filled circles), together with the HMAbs 23e.

Xiang, S. H., Doka, N., Choudhary, R. K., Sodroski, J., & Robinson, J. E. (2002). Characterization of CD4-induced epitopes on the HIV type 1 gp120 envelope glycoprotein recognized by neutralizing human monoclonal antibodies. AIDS research and human retroviruses, 18(16), 1207-1217.

Figure 2 Ability of CD4i HMAbs to compete with 17b antibody for gp120. The HIV-1 gp120 glycoprotein was captured on ELISA plates with the D7324 antibody.

The binding of biotinylated 17b antibody to the gp120 glycoprotein in the presence of the indicated concentrations of competitor HMAbs was detected by avidin-peroxidase.

Xiang, S. H., Doka, N., Choudhary, R. K., Sodroski, J., & Robinson, J. E. (2002). Characterization of CD4-induced epitopes on the HIV type 1 gp120 envelope glycoprotein recognized by neutralizing human monoclonal antibodies. AIDS research and human retroviruses, 18(16), 1207-1217.

Figure 3 Ability of CD4i HMAbs to inhibit gp120-CCR5 binding.

Radiolabeled gp120 was mixed with sCD4 and then incubated with Cf2ThsynCCR5 cells in the presence of the indicated concentrations of CD4i HMAbs.

Xiang, S. H., Doka, N., Choudhary, R. K., Sodroski, J., & Robinson, J. E. (2002). Characterization of CD4-induced epitopes on the HIV type 1 gp120 envelope glycoprotein recognized by neutralizing human monoclonal antibodies. AIDS research and human retroviruses, 18(16), 1207-1217.

Figure 4 Soluble CD4 and CD4i HMAbs exhibit positive cooperativity in precipitating HIV-1 gp120 glycoprotein mutants.

The radiolabeled 257 T/D mutant was precipitated by CD4-Ig, 17b, or a combination of sCD4 and 17b. The 257 T/D mutant was also precipitated by a mixture of sera from HIV-1-infected individuals (Pt. sera). The precipitated protein was analyzed by SDS-PAGE and autoradiography.

Xiang, S. H., Doka, N., Choudhary, R. K., Sodroski, J., & Robinson, J. E. (2002). Characterization of CD4-induced epitopes on the HIV type 1 gp120 envelope glycoprotein recognized by neutralizing human monoclonal antibodies. AIDS research and human retroviruses, 18(16), 1207-1217.

Figure 5 Neutralization of HIV-1 by CD4i HMAbs.

Recombinant HIV-1 expressing luciferase and containng env deletions were pseudotyped with HIV-1 envelope glycoproteins from the HXBc2, MN, ADA, JR-FL, and 89.6 isolates. The viruses were incubated with the indicated concentration of HMAb for 1 hr at 37°C prior to exposure to the appropriate target cells.

Xiang, S. H., Doka, N., Choudhary, R. K., Sodroski, J., & Robinson, J. E. (2002). Characterization of CD4-induced epitopes on the HIV type 1 gp120 envelope glycoprotein recognized by neutralizing human monoclonal antibodies. AIDS research and human retroviruses, 18(16), 1207-1217.

Figure 6 The sulfated CCR5 peptide interacts with a CD4-induced epitope of gp120 that includes tropism determinants in the V3 and C4 regions of gp120.

The gp120 glycoprotein of the R5 isolate ADA was incubated with the indicated antibodies in the presence of peptide-free buffer, the C-peptide (gray bars), or the S-peptide (black bars). The mixtures were then precipitated with the indicated antibodies, and precipitates were analyzed by SDS-PAGE. Precipitation by the 17b, A32, 48d, and 23e antibodies.

Farzan, M., Vasilieva, N., Schnitzler, C. E., Chung, S., Robinson, J., Gerard, N. P., ... & Sodroski, J. (2000). A tyrosine-sulfated peptide based on the N terminus of CCR5 interacts with a CD4-enhanced epitope of the HIV-1 gp120 envelope glycoprotein and inhibits HIV-1 entry. Journal of Biological Chemistry, 275(43), 33516-33521.