Mouse Anti-HMGB1 Recombinant Antibody (clone 3E8) (CAT#: HPAB-0215-FY)
This product is a recombinant antibody that recognizes Human HMGB1. The monoclonal antibody 3E 8 reacts with the epitope of HMGB1.
Specific Inquiry
WB
Figure 1 Generation of high affinity, biologically active anti-HMGB1 antibodies from NZB/W mice.
A T cell-specific tag fused to HMGB1 further increased immune response in NZB/W mice. NZB/W mice were immunized with GST-HMGB1 or HMGB1-MT.
Zhou, H., Wang, Y., Wang, W., Jia, J., Li, Y., Wang, Q., ... & Tang, J. (2009). Generation of monoclonal antibodies against highly conserved antigens. PLoS One, 4(6), e6087.
Block
Figure 2 Anti-HMGB1 antibodies blocked HMGB1-induced IL-6 up-regulation.
Data are expressed as the means±SEM (n = 3).
Zhou, H., Wang, Y., Wang, W., Jia, J., Li, Y., Wang, Q., ... & Tang, J. (2009). Generation of monoclonal antibodies against highly conserved antigens. PLoS One, 4(6), e6087.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HMGB1
- Species Reactivity
- Human
- Clone
- 3E8
- Applications
- Block, WB
- Related Disease
- Sepsis, systemic inflammatory response syndrome or acute lung injury, the autoimmune disease
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HMGB1 antibody has been reported in applications of Western Blot, Blocking. WB: Raw264.7 cells were stimulated with 1 µg/ml HMGB1 for 8 hours, with or without 50 µg/ml of the antibodies indicated.
Target
- Alternative Names
- High Mobility Group Box 1; High-Mobility Group (Nonhistone Chromosomal) Protein 1; Sulfoglucuronyl Carbohydrate Binding Protein; High Mobility Group Protein 1; Amphoterin; HMG-1
- Gene ID
- 3146
- UniProt ID
- P09429
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone 3E8"
See other products for "HMGB1"
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0315CL | Mouse Anti-HMGB1 Recombinant Antibody (TAB-0315CL) | IHC | Mouse IgG |
| TAB-0316CL | Mouse Anti-HMGB1 Recombinant Antibody (TAB-0316CL) | ELISA, Inhib, FuncS, WB | Mouse IgG |
| TAB-0317CL | Mouse Anti-HMGB1 Recombinant Antibody (TAB-0317CL) | ELISA, Inhib, FuncS, WB | Mouse IgG |
| TAB-0318CL | Mouse Anti-HMGB1 Recombinant Antibody (TAB-0318CL) | ELISA, Inhib, FuncS, WB | Mouse IgG |
| TAB-0315CL-S(P) | Mouse Anti-HMGB1 Recombinant Antibody; scFv Fragment (TAB-0315CL-S(P)) | ELISA, Inhib, FuncS, WB | Mouse scFv |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-099CT | Human Anti-HMGB1 Recombinant Antibody (TAB-099CT) | IF, ELISA, IHC | Human IgG1, λ |
| TAB-099CT-S(P) | Human Anti-HMGB1 Recombinant Antibody; scFv Fragment (TAB-099CT-S(P)) | ELISA, FC, Block | Human scFv |
| TAB-099CT-F(E) | Human Anti-HMGB1 Recombinant Antibody; Fab Fragment (TAB-099CT-F(E)) | ELISA, FC, Block | Humanized Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-101CT | Anti-Human HMG1 Recombinant Antibody | ELISA, BL | Human antibody |
| TAB-101CT-S(P) | Anti-Human HMG1 Recombinant Antibody scFv Fragment | ELISA, BL | Human antibody |
| TAB-101CT-F(E) | Anti-Human HMG1 Recombinant Antibody Fab Fragment | ELISA, BL | Human antibody |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0839CT | Recombinant Mouse anti-Human HMGB1 Monoclonal antibody (19N10B7) | ELISA, FC, ICC/IF, IHC-P, WB | |
| MRO-0755-CN | Rabbit Anti-HMGB1 Recombinant Antibody (clone CBACN-279) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1938-CN | Rabbit Anti-HMGB1 Polyclonal Antibody (MRO-1938-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1939-CN | Rabbit Anti-HMGB1 Polyclonal Antibody (MRO-1939-CN) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-N0137-YC | Mouse Anti-HMGB1 Recombinant Antibody (HPAB-N0137-YC) | ELISA, SPR | Mouse IgG2a |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1633 | Rabbit Anti-HMGB1 Recombinant Antibody (clone DS1633AB) | WB, IHC-P, FC | Rabbit IgG |
| MOR-0049-FY | Rabbit Anti-HMGB1 Recombinant Antibody (clone AFY0020) | ICC, IHC-P, WB | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2082 | PE-B*07:02/Human HMGB1 (APKRPPSAF) MHC Tetramer | FCM | |
| MHC-LC2083 | APC-B*07:02/Human HMGB1 (APKRPPSAF) MHC Tetramer | FCM |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0215-FY-S(P) | Mouse Anti-HMGB1 Recombinant Antibody (clone 3E8); scFv Fragment | ELISA | Mouse scFv |
| HPAB-N0137-YC-S(P) | Mouse Anti-HMGB1 Recombinant Antibody; scFv Fragment (HPAB-N0137-YC-S(P)) | ELISA | Mouse scFv |
| HPAB-0101-WJ-S(P) | Human Anti-HMGB1 Recombinant Antibody; scFv Fragment (HPAB-0101-WJ-S(P)) | ELISA, WB | Humanized scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0215-FY-F(E) | Mouse Anti-HMGB1 Recombinant Antibody (clone 3E8); Fab Fragment | ELISA | Mouse Fab |
| HPAB-N0137-YC-F(E) | Mouse Anti-HMGB1 Recombinant Antibody; Fab Fragment (HPAB-N0137-YC-F(E)) | ELISA | Mouse Fab |
| HPAB-0101-WJ-F(E) | Human Anti-HMGB1 Recombinant Antibody; Fab Fragment (HPAB-0101-WJ-F(E)) | ELISA, WB | Humanized Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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