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Mouse Anti-HMGB1 Recombinant Antibody (clone 3E8); scFv Fragment (CAT#: HPAB-0215-FY-S(P))

Recombinant scFv fragment to HMGB1. This product is a recombinant antibody that recognizes Human HMGB1. The monoclonal antibody 3E 8 reacts with the epitope of HMGB1.


See more Engineered Antibody products for HMGB1
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  • Purity:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73663/2043_F12_32_selected.jpg
Figure 2 IF staining of human cell line A-431
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/617_A1_1_selected.jpg
Figure 3 IHC staining of human bone marrow
Figure 3 IHC staining of human bone marrow
(Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_4_4_selected.jpg
Figure 4 IHC staining of human esophagus
Figure 4 IHC staining of human esophagus
(Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/ihc_selected.jpg
Figure 5 IF staining of human cell line U-2 OS
Figure 5 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/613_A1_1_red_green.jpg
Figure 6 IF staining of human cell line U-251 MG
Figure 6 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/610_A1_1_red_green.jpg
Normal Tissue
Figure 7 Cerebral cortex
Figure 7 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_8_5.jpg
Figure 8 Colon
Figure 8 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_9_3.jpg
Figure 9 Liver
Figure 9 Liver
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_4.jpg
Figure 10 Kidney
Figure 10 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_7_5.jpg
Figure 11 Lymph node
Figure 11 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1
Figure 12 RNA cell line category: Low cell line specificity
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1

Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse scFv
  • Specificity
  • Human HMGB1
  • Species Reactivity
  • Human
  • Clone
  • 3E8
  • Applications
  • ELISA
  • Related Disease
  • Sepsis, systemic inflammatory response syndrome or acute lung injury, the autoimmune disease

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • High Mobility Group Box 1; High-Mobility Group (Nonhistone Chromosomal) Protein 1; Sulfoglucuronyl Carbohydrate Binding Protein; High Mobility Group Protein 1; Amphoterin; HMG-1

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone 3E8"

See other products for "HMGB1"

Mouse Antibody

CAT Product Name Application Type
TAB-0315CL Mouse Anti-HMGB1 Recombinant Antibody (TAB-0315CL) IHC Mouse IgG
TAB-0316CL Mouse Anti-HMGB1 Recombinant Antibody (TAB-0316CL) ELISA, Inhib, FuncS, WB Mouse IgG
TAB-0317CL Mouse Anti-HMGB1 Recombinant Antibody (TAB-0317CL) ELISA, Inhib, FuncS, WB Mouse IgG
TAB-0318CL Mouse Anti-HMGB1 Recombinant Antibody (TAB-0318CL) ELISA, Inhib, FuncS, WB Mouse IgG
TAB-100CT Anti-Human HMG1 Recombinant Antibody (DPH1 .1 mAb) IF, FC

Humanized Antibody

CAT Product Name Application Type
TAB-099CT Human Anti-HMGB1 Recombinant Antibody (TAB-099CT) IF, ELISA, IHC Human IgG1, λ
TAB-099CT-S(P) Human Anti-HMGB1 Recombinant Antibody; scFv Fragment (TAB-099CT-S(P)) ELISA, FC, Block Human scFv
TAB-099CT-F(E) Human Anti-HMGB1 Recombinant Antibody; Fab Fragment (TAB-099CT-F(E)) ELISA, FC, Block Humanized Fab

Human Antibody

CAT Product Name Application Type
TAB-101CT Anti-Human HMG1 Recombinant Antibody ELISA, BL Human antibody
TAB-101CT-S(P) Anti-Human HMG1 Recombinant Antibody scFv Fragment ELISA, BL Human antibody
TAB-101CT-F(E) Anti-Human HMG1 Recombinant Antibody Fab Fragment ELISA, BL Human antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1633 Rabbit Anti-HMGB1 Recombinant Antibody (clone DS1633AB) WB, IHC-P, FC Rabbit IgG
MOR-0049-FY Rabbit Anti-HMGB1 Recombinant Antibody (clone AFY0020) ICC, IHC-P, WB Rabbit IgG

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC2082 PE-B*07:02/Human HMGB1 (APKRPPSAF) MHC Tetramer FCM
MHC-LC2083 APC-B*07:02/Human HMGB1 (APKRPPSAF) MHC Tetramer FCM

Recombinant Antibody

CAT Product Name Application Type
MRO-0755-CN Rabbit Anti-HMGB1 Recombinant Antibody (clone CBACN-279) WB, IF, IHC, FC Rabbit IgG
MRO-1938-CN Rabbit Anti-HMGB1 Polyclonal Antibody (MRO-1938-CN) WB, IF, IHC, FC Rabbit IgG
MRO-1939-CN Rabbit Anti-HMGB1 Polyclonal Antibody (MRO-1939-CN) WB, IF, IHC, FC Rabbit IgG
HPAB-0215-FY Mouse Anti-HMGB1 Recombinant Antibody (clone 3E8) Block, WB Mouse IgG
ZG-0740J Rabbit Anti-HMGB1 Recombinant Antibody (clone 1A1) ELISA, WB, IHC Rabbit IgG

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0215-FY-F(E) Mouse Anti-HMGB1 Recombinant Antibody (clone 3E8); Fab Fragment ELISA Mouse Fab
HPAB-N0137-YC-F(E) Mouse Anti-HMGB1 Recombinant Antibody; Fab Fragment (HPAB-N0137-YC-F(E)) ELISA Mouse Fab
HPAB-0101-WJ-F(E) Human Anti-HMGB1 Recombinant Antibody; Fab Fragment (HPAB-0101-WJ-F(E)) ELISA, WB Humanized Fab

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-N0137-YC-S(P) Mouse Anti-HMGB1 Recombinant Antibody; scFv Fragment (HPAB-N0137-YC-S(P)) ELISA Mouse scFv
HPAB-0101-WJ-S(P) Human Anti-HMGB1 Recombinant Antibody; scFv Fragment (HPAB-0101-WJ-S(P)) ELISA, WB Humanized scFv

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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