Rabbit Anti-HSPA5 Recombinant Antibody (clone 4H12) (CAT#: ZG-0749J)

This product is a rabbit antibody that recognizes HSPA5.


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  • Tested Data
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  • Datasheet
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  • COA
IHC

Figure 1 Rabbit Anti-HSPA5 Antibody (ZG-0749J) in IHC

Figure 1 Rabbit Anti-HSPA5 Antibody (ZG-0749J) in IHC

IHC image of this product diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.


Specifications

  • Immunogen
  • A synthesized peptide derived from human GRP78 BiP
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human HSPA5
  • Species Reactivity
  • Human
  • Clone
  • 4H12
  • Applications
  • ELISA, IHC
  • Conjugate
  • Uconjugated

Product Property

  • Purification
  • Affinity purified
  • Format
  • Liquid
  • Concentration
  • See the COA
  • Buffer
  • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
    IHC:1:50-1:200

Target

  • Alternative Names
  • BIP; MIF2; GRP78; HEL-S-89n; endoplasmic reticulum chaperone BiP; 78 kDa glucose-regulated protein; HSP70 family protein 5; binding-immunoglobulin protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; glucose-regulated protein, 78kDa; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); heat shock protein 70 family protein 5; heat shock protein family A member 5; immunoglobulin heavy chain-binding protein

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "HSPA5"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1695 Rabbit Anti-HSPA5 Recombinant Antibody (clone DS1695AB) WB, IHC-P Rabbit IgG

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-0368-YJ-S(P) Mouse Anti-HSPA5 Recombinant Antibody (clone MAb159); scFv Fragment WB, FC, IHC, ELISA, Inhib, FuncS Mouse scFv
HPAB-0369-YJ-S(P) Human Anti-HSPA5 Recombinant Antibody (clone huMAb159); scFv Fragment WB, FC, IHC, ELISA, Inhib, FuncS Humanized scFv

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0368-YJ-F(E) Mouse Anti-HSPA5 Recombinant Antibody (clone MAb159); Fab Fragment WB, FC, IHC, ELISA, Inhib, FuncS Mouse Fab
HPAB-0369-YJ-F(E) Human Anti-HSPA5 Recombinant Antibody (clone huMAb159); Fab Fragment WB, FC, IHC, ELISA, Inhib, FuncS Humanized Fab

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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