Human Anti-IgE Recombinant Antibody (clone Omaizumab S267E/L328F) (CAT#: HPAB-AP674-YC)
Provided is a immunoglobulin that binds IgE with high affinity. The antibody is capable of inhibiting cells that express membrane-anchored IgE. Also, the antibody is useful for treating IgE-mediated disorders, including allergies and asthma.
Specific Inquiry
ELISA
Figure 1 Inhibition of IgE binding to FcεRIα through ligelizumab or omalizumab IgG by ELISA.
Inhibition of IgE binding to FcεRIα and CD23 by ligelizumab or omalizumab.
Gasser, P., Tarchevskaya, S. S., Guntern, P., Brigger, D., Ruppli, R., Zbären, N., ... & Eggel, A. (2020). The mechanistic and functional profile of the therapeutic anti-IgE antibody ligelizumab differs from omalizumab. Nature communications, 11(1), 1-14.
Inhib
Figure 2 Isolated primary human blood basophils are gated as SSClow, FSClow, CD123+, CD193+ double-positive cells.
After resensitization with recombinant human JW8-IgE (dashed gray line) is shown.
Gasser, P., Tarchevskaya, S. S., Guntern, P., Brigger, D., Ruppli, R., Zbären, N., ... & Eggel, A. (2020). The mechanistic and functional profile of the therapeutic anti-IgE antibody ligelizumab differs from omalizumab. Nature communications, 11(1), 1-14.
FC
Figure 3 Representative flow cytometry plots and quantification of IgG-positive RPMI8866 cell frequencies.
Representative image stream flow cytometry pictures for IgE staining.
Gasser, P., Tarchevskaya, S. S., Guntern, P., Brigger, D., Ruppli, R., Zbären, N., ... & Eggel, A. (2020). The mechanistic and functional profile of the therapeutic anti-IgE antibody ligelizumab differs from omalizumab. Nature communications, 11(1), 1-14.
IHC
Figure 4 Co-localization analysis for IgE staining of IgE-sensitized RPMI8866 cells.
Binding of CD23:IgE complexes by ligelizumab or omalizumab.
Gasser, P., Tarchevskaya, S. S., Guntern, P., Brigger, D., Ruppli, R., Zbären, N., ... & Eggel, A. (2020). The mechanistic and functional profile of the therapeutic anti-IgE antibody ligelizumab differs from omalizumab. Nature communications, 11(1), 1-14.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG1
- Specificity
- Human IgE
- Species Reactivity
- Human
- Clone
- Omaizumab S267E/L328F
- Applications
- Inhib, FC, IHC, ELISA
- Related Disease
- Allergies and Asthma
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The IgE antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Flow Cytometry, Inhibition, Immunohistochemistry.
Target
- Alternative Names
- Immunoglobulin E
Related Resources
Asthma
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-034ZJ | Anti-Human IgE Recombinant Antibody (CMAB007) | ELISA | Humanized antibody |
| TAB-049ZJ | Anti-Human IgE Recombinant Antibody (IGE009) | ELISA, Inhib, FC | Humanized antibody |
| TAB-034ZJ-S(P) | Anti-Human IgE Recombinant Antibody scFv Fragment (CMAB007) | ELISA | Humanized antibody |
| TAB-049ZJ-S(P) | Anti-Human IgE Recombinant Antibody scFv Fragment (IGE009) | ELISA, Inhib, FC | Humanized antibody |
| TAB-050ZJ-S(P) | Anti-Human IgE Recombinant Antibody scFv Fragment (IGE026) | ELISA, Inhib, FC | Humanized antibody |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-095ZJ | Anti-Human IgE Therapeutic Single Domain Antibody (TAB-095ZJ) | ELISA, Inhib, FC | Single domain antibody |
Fab Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-009LC | Recombinant Anti-Human IgE Antibody (Fab glycosylation/Fc glycosylation) | ELISA, IP | Human antibody |
| Gly-010LC | Recombinant Anti-Human IgE Antibody (Fab glycosylation/Fc glycosylation) | ELISA, IP | Human antibody |
| Gly-011LC | Recombinant Anti-Human IgE Antibody (Fab glycosylation/Fc glycosylation) | ELISA, IP | Human antibody |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-009LC-1 | Recombinant Anti-Human IgE Antibody (Fab glycosylation/Fc glycosylation) | ELISA, IP | Human antibody |
| Gly-010LC-1 | Recombinant Anti-Human IgE Antibody (Fab glycosylation/Fc glycosylation) | ELISA, IP | Human antibody |
| Gly-011LC-1 | Recombinant Anti-Human IgE Antibody (Fab glycosylation/Fc glycosylation) | ELISA, IP | Human antibody |
| Gly-156LC | Recombinant Anti-Human IgE Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
Low- or Non-fucosylated Oligosaccharide
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-156LC-1 | Recombinant Anti-Human IgE Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0043-WJ | Mouse Anti-IgE Recombinant Antibody (HPAB-0043-WJ) | ELISA, WB, FC, ADCC, FuncS | Mouse IgG |
| HPAB-N0143-YC | Human Anti-IgE Recombinant Antibody (clone hu4F11-12) | ELISA, Inhib | Humanized IgG1, κ |
| HPAB-0283-YJ | Mouse Anti-IgE Recombinant Antibody (HPAB-0283-YJ) | ELISA, Enzymatic activity | Mouse IgG |
| HPAB-1281WJ | Canine Anti-IgE Recombinant Antibody (clone c15A.2) | Inhib, ELISA | Chimeric (mouse/canine) IgG |
| FAMAB-1380CQ | Rat Anti-IgE Recombinant Antibody (clone R1E4) | FC, FuncS, ELISA | Rat IgG1 |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP674-YC-S(P) | Human Anti-IgE Recombinant Antibody (clone Omaizumab S267E/L328F); scFv Fragment | ELISA, FuncS | Human scFv |
| HPAB-AP675-YC-S(P) | Human Anti-IgE Recombinant Antibody (clone MaE11-H1L1); scFv Fragment | ELISA, FuncS | Human scFv |
| HPAB-AP676-YC-S(P) | Human Anti-IgE Recombinant Antibody (clone MaE11-H1L1 S267E/L328F); scFv Fragment | ELISA, FuncS | Human scFv |
| HPAB-1574WJ-S(P) | Human Anti-IgE Recombinant Antibody; scFv Fragment (clone HuMAE11V1) | ELISA, WB, FC | Human scFv |
| HPAB-1600WJ-S(P) | Mouse Anti-IgE Recombinant Antibody; scFv Fragment (clone e426) | ELISA, WB | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP674-YC-F(E) | Human Anti-IgE Recombinant Antibody (clone Omaizumab S267E/L328F); Fab Fragment | ELISA, FuncS | Humanized Fab |
| HPAB-AP675-YC-F(E) | Human Anti-IgE Recombinant Antibody (clone MaE11-H1L1); Fab Fragment | ELISA, FuncS | Humanized Fab |
| HPAB-AP676-YC-F(E) | Human Anti-IgE Recombinant Antibody (clone MaE11-H1L1 S267E/L328F); Fab Fragment | ELISA, FuncS | Humanized Fab |
| HPAB-AP677-YC-F(E) | Human Anti-IgE Recombinant Antibody (clone MaE11-H1L1 G236R/L328F); Fab Fragment | ELISA, FuncS | Humanized Fab |
| HPAB-AP678-YC-F(E) | Mouse Anti-IgE Recombinant Antibody (clone MaE11); Fab Fragment | ELISA, Inhib | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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