Rat Anti-IgE Recombinant Antibody (clone R1E4) (CAT#: FAMAB-1380CQ)
This product is a recombinant rat antibody that recognizes IgE. The antibody was purified by affinity chromatography. It can be used in ELISA, FC.
Specific Inquiry
FC
Figure 1 Generation and characterization of recombinant neutralizing anti-IgE single-chain Ab construct.
Recombinant plasmids expressing the membrane form of single-chain R1E4 were transiently cotransfected with enhanced GFP (EGFP) plasmid into 293 cells and the ability of the cells to bind to IgE was analyzed by flow cytometry.
Ota, T., Aoki-Ota, M., Duong, B. H., & Nemazee, D. (2020). Suppression of IgE B Cells and IgE Binding to FcεRI by Gene Therapy with Single-Chain Anti-IgE. The Journal of Immunology, 8110-8117.
FuncS
Figure 2 Effect of in vivo expression of secreted form of chimeric single-chain anti-IgE on markers of IgE expression.
Total serum IgE concentration before treatment (□) and 13 days after treatment (■).
Ota, T., Aoki-Ota, M., Duong, B. H., & Nemazee, D. (2020). Suppression of IgE B Cells and IgE Binding to FcεRI by Gene Therapy with Single-Chain Anti-IgE. The Journal of Immunology, 8110-8117.
ELISA
Figure 3 Ability of rR1E4 anti-IgE protein to affect the biological responses of IgE+ B cells generated in tissue culture.
IgE levels in culture supernatants on day 4 as measured by ELISA.
Ota, T., Aoki-Ota, M., Duong, B. H., & Nemazee, D. (2020). Suppression of IgE B Cells and IgE Binding to FcεRI by Gene Therapy with Single-Chain Anti-IgE. The Journal of Immunology, 8110-8117.
Specifications
- Immunogen
- Mouse IgE
- Host Species
- Rat
- Type
- Rat IgG1
- Specificity
- Mouse IgE
- Species Reactivity
- Mouse
- Clone
- R1E4
- Applications
- FC, FuncS, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Flow Cytometry, Enzyme-Linked Immunosorbent Assay and Function Assay.
Target
- Alternative Names
- Immunoglobulin E; IgE
- UniProt ID
- P06336
Related Resources
Asthma
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-034ZJ | Anti-Human IgE Recombinant Antibody (CMAB007) | ELISA | Humanized antibody |
| TAB-049ZJ | Anti-Human IgE Recombinant Antibody (IGE009) | ELISA, Inhib, FC | Humanized antibody |
| TAB-050ZJ | Anti-Human IgE Recombinant Antibody (IGE026) | ELISA, Inhib, FC | Humanized antibody |
| TAB-034ZJ-F(E) | Anti-Human IgE Recombinant Antibody Fab Fragment (CMAB007) | ELISA | Humanized antibody |
| TAB-049ZJ-F(E) | Anti-Human IgE Recombinant Antibody Fab Fragment (IGE009) | ELISA, Inhib, FC | Humanized antibody |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-095ZJ | Anti-Human IgE Therapeutic Single Domain Antibody (39D11) | ELISA, Inhib, FC | Single domain antibody |
Fab Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-009LC | Recombinant Anti-Human IgE Antibody (Fab glycosylation/Fc glycosylation) | ELISA, IP | Human antibody |
| Gly-010LC | Recombinant Anti-Human IgE Antibody (Fab glycosylation/Fc glycosylation) | ELISA, IP | Human antibody |
| Gly-011LC | Recombinant Anti-Human IgE Antibody (Fab glycosylation/Fc glycosylation) | ELISA, IP | Human antibody |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-009LC-1 | Recombinant Anti-Human IgE Antibody (Fab glycosylation/Fc glycosylation) | ELISA, IP | Human antibody |
| Gly-010LC-1 | Recombinant Anti-Human IgE Antibody (Fab glycosylation/Fc glycosylation) | ELISA, IP | Human antibody |
| Gly-011LC-1 | Recombinant Anti-Human IgE Antibody (Fab glycosylation/Fc glycosylation) | ELISA, IP | Human antibody |
| Gly-156LC | Recombinant Anti-Human IgE Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
Low- or Non-fucosylated Oligosaccharide
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-156LC-1 | Recombinant Anti-Human IgE Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0209CQ-S(P) | Human Anti-IgE Recombinant Antibody (clone huTES-C21); scFv Fragment | ELISA, Block | Humanized scFv |
| HPAB-0043-WJ-S(P) | Mouse Anti-IgE Recombinant Antibody; scFv Fragment (HPAB-0043-WJ-S(P)) | ELISA, WB | Mouse scFv |
| HPAB-AP102-YC-S(P) | Mouse Anti-IgE Recombinant Antibody (clone 47H4); scFv Fragment | ELISA | Mouse scFv |
| HPAB-AP103-YC-S(P) | Mouse Anti-IgE Recombinant Antibody (clone 7A6); scFv Fragment | ELISA | Mouse scFv |
| HPAB-AP104-YC-S(P) | Mouse Anti-IgE Recombinant Antibody (clone 26A11); scFv Fragment | ELISA | Mouse scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0043-WJ | Mouse Anti-IgE Recombinant Antibody (HPAB-0043-WJ) | ELISA, WB, FC, ADCC, FuncS | Mouse IgG |
| HPAB-AP102-YC | Mouse Anti-IgE Recombinant Antibody (clone 47H4) | ELISA | Mouse IgG |
| HPAB-AP103-YC | Mouse Anti-IgE Recombinant Antibody (clone 7A6) | WB, FuncS, ELISA | Mouse IgG |
| HPAB-AP104-YC | Mouse Anti-IgE Recombinant Antibody (clone 26A11) | ELISA | Mouse IgG |
| HPAB-AP105-YC | Human Anti-IgE Recombinant Antibody (clone 47H4v5) | ELISA | Human IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP102-YC-F(E) | Mouse Anti-IgE Recombinant Antibody (clone 47H4); Fab Fragment | ELISA | Mouse Fab |
| HPAB-AP103-YC-F(E) | Mouse Anti-IgE Recombinant Antibody (clone 7A6); Fab Fragment | ELISA | Mouse Fab |
| HPAB-AP104-YC-F(E) | Mouse Anti-IgE Recombinant Antibody (clone 26A11); Fab Fragment | ELISA | Mouse Fab |
| HPAB-AP105-YC-F(E) | Human Anti-IgE Recombinant Antibody (clone 47H4v5); Fab Fragment | ELISA | Human Fab |
| HPAB-1195-FY-S(P) | Human Anti-IgE Recombinant Antibody; scFv Fragment (HPAB-1195-FY-S(P)) | ELISA | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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