Recombinant Mouse Anti-JEV Antibody (2H4) (CAT#: PABC-365)

Figure 1 A representative SDS-PAGE analysis result of the Co-IP experiments.

Vero membrane protein extracts were precipitated with JEV and anti-JEV mAb 2H4, and subjected to SDS-PAGE under non-reducing conditions. Three novel bands at approximately 43, 55 and 95 kDa (indicated by red arrows) in the Co-IP lane were noticed, and the gels were excised for mass spectrometry analyses.

Wang, Y., Li, Y., & Ding, T. (2017). Heat shock protein 90β in the Vero cell membrane binds Japanese encephalitis virus. International journal of molecular medicine, 40(2), 474-482.

Figure 2 2F2 and 2H4 neutralize JEV by inhibiting attachment and fusion of JEV.

a, The amount of virions on the cell surface was detected by RT-PCR after exposure to 2F2 or 2H4, before (left) and after (right) the virus was allowed to attach to cells. High concentrations of 2F2 and 2H4 prevented attachment of JEV to the cell surface when JEV was exposed to antibodies before cell attachment. b, 2F2 and 2H4 block low-pH-induced fusion of JEV with liposomes. The liposomes were loaded with self-quenching concentrations of the fluorescent dye calcein. Fusion of JEV with liposomes occurred at pH 5.6.

Qiu, X., Lei, Y., Yang, P., Gao, Q., Wang, N., Cao, L., ... & Ding, T. (2018). Structural basis for neutralization of Japanese encephalitis virus by two potent therapeutic antibodies. Nature microbiology, 3(3), 287.