Rabbit Anti-KIF2C Recombinant Antibody (clone R06-2D7) (CAT#: VS3-FY2029)
This product is a rabbit recombinant monoclonal antibody. It is designed to target human KIF2C. It is suitable for use in a variety of assays, including WB, ICC/IF. We detected the antibody in the immunocytochemistry analysis of MCAK in Jurkat cell.
Specific Inquiry
WB
Figure 1 Anti-KIF2C Antibody (VS3-FY2029) in WB.
The MCAK expression in Hela, CHO-K1, Ramos lysates were detected using the anti-MCAK by western blot assay.
ICC
Figure 2 Anti-KIF2C Antibody (VS3-FY2029) in ICC.
Immunocytochemistry staining of MCAK (green) in Jurkat using the anti-MCAK antibody. Nuclear DNA was labeled with DAPI (shown in blue).
Specifications
- Immunogen
- Synthetic peptide of human MCAK
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human KIF2C
- Species Reactivity
- Human
- Clone
- R06-2D7
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 81 kDa, while the observed molecular weight stands at 81 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Kinesin Family Member 2C; Mitotic Centromere-Associated Kinesin; Kinesin-Like Protein 6; KNSL6; MCAK; Kinesin-Like 6 (Mitotic Centromere-Associated Kinesin); Testis Tissue Sperm-Binding Protein Li 68n; Kinesin-Like Protein KIF2C; CT139
- Gene ID
- 11004
- UniProt ID
- Q99661
- Sequence Similarities
- Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. MCAK/KIF2 subfamily.
- Cellular Localization
- Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore
- Post Translation Modifications
- Phosphorylation by AURKB, regulates association with centromeres and kinetochores and the microtubule depolymerization activity. (Q99661-KIF2C_HUMAN)
Ubiquitinated. (Q99661-KIF2C_HUMAN)
Ubiquitination at Lys38, Lys365, and Lys518 (NX_Q99661 [NX_Q99661-1])
Modification sites at PhosphoSitePlus (Q99661)
Glycosylation from GlyGen (Q99661) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047297658.1; XP_047297664.1; XP_054189932.1; XP_054189933.1; NP_001284584.1
- Function
- Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "KIF2C"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1123CT | Recombinant Mouse anti-Human KIF2C Monoclonal antibody (3599D4b) | Dot, WB |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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