Human Anti-KRT8 Recombinant Antibody (clone COU-1) (CAT#: FAMAB-0344JF)
This recombinant monoclonal antibody specially binds to modified cytokeratin 8 (KRT8) expressed on the surface of carcinoma cells. This antibody is a human monoclonal antibody that can be used in ELISA, WB, and IHC.
Specific Inquiry
WB
Figure 1 Western blots showing KRT8 protein expression in tumor tissues.
KRT8 upregulation in primary metastatic ccRCC tissues.
Tan, H. S., Jiang, W. H., He, Y., Wang, D. S., Wu, Z. J., Wu, D. S., ... & Ma, L. J. (2017). KRT8 upregulation promotes tumor metastasis and is predictive of a poor prognosis in clear cell renal cell carcinoma. Oncotarget, 8(44), 76189.
IHC
Figure 2 IHC characteristics of KRT8 in ccRCC and adjacent NT tissues.
Representative staining results for KRT8 are shown.
Tan, H. S., Jiang, W. H., He, Y., Wang, D. S., Wu, Z. J., Wu, D. S., ... & Ma, L. J. (2017). KRT8 upregulation promotes tumor metastasis and is predictive of a poor prognosis in clear cell renal cell carcinoma. Oncotarget, 8(44), 76189.
ELISA
Figure 3 Western blot analyses of KRT8 in WT human osteoblasts (hOb) compared to hOb isolated from a rib of a 24 year old female homozygotic F508del-CFTR patient.
F508del-Cftr hOb compared to HeLa cells was used as positive controls.
Le Henaff, C., Faria Da Cunha, M., Hatton, A., Tondelier, D., Marty, C., Collet, C., ... & Edelman, A. (2016). Genetic deletion of keratin 8 corrects the altered bone formation and osteopenia in a mouse model of cystic fibrosis. Human Molecular Genetics, 25(7), 1281-1293.
FC
Figure 4 Inhibition of autophagy increases apoptotic cell death in RPE cells under oxidative stress.
Human primary RPE cells were stained with FITC-conjugated ANXA5 and propidium iodide (PI) and analyzed by FACS.
Baek, A., Yoon, S., Kim, J., Baek, Y. M., Park, H., Lim, D., ... & Kim, D. E. (2017). Autophagy and KRT8/keratin 8 protect degeneration of retinal pigment epithelium under oxidative stress. Autophagy, 13(2), 248-263.
Specifications
- Host Species
- Human
- Type
- Human IgM
- Specificity
- Human KRT8, KRT18
- Species Reactivity
- Human
- Clone
- COU-1
- Applications
- WB, ELISA, IHC, FC
- Related Disease
- Colon carcinomas, Rectal carcinomas, Pancreatic carcinomas
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The KRT8 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Flow Cytometry.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1511MZ | Recombinant Mouse Anti-Human KRT8 Antibody (clone 2C23) | ICC/IF, WB | Mouse antibody |
| HPAB-0199-WJ | Mouse Anti-KRT8 Recombinant Antibody (clone 8G8) | ELISA, IHC | Mouse IgG1 |
| ZG-0471F | Mouse Anti-KRT8 Recombinant Antibody (ZG-0471F) | WB, IHC, IF, ELISA | Mouse IgG |
| ZG-0984F | Mouse Anti-KRT8 Recombinant Antibody (clone 35βH11) | IHC-Fr, IHC-P, WB | Mouse IgM |
| ZG-1046F | Mouse Anti-KRT8 Recombinant Antibody (clone M20) | FC, ICC, IHC-Fr, IHC-P, WB | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1993 | Hi-Affi™ Recombinant Rabbit Anti-KRT8 Monoclonal Antibody (DS1993AB) | IHC-P, IHC-Fr | IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0199-WJ-S(P) | Mouse Anti-KRT8 Recombinant Antibody (clone 8G8); scFv Fragment | ELISA, IHC | Mouse scFv |
| FAMAB-0344JF-S(P) | Human Anti-KRT8 Recombinant Antibody (clone COU-1); scFv Fragment | IHC, ELISA, FuncS | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0199-WJ-F(E) | Mouse Anti-KRT8 Recombinant Antibody (clone 8G8); Fab Fragment | ELISA, IHC | Mouse Fab |
| FAMAB-0344JF-F(E) | Human Anti-KRT8 Recombinant Antibody (clone COU-1); Fab Fragment | IHC, ELISA, FuncS | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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