Rabbit Anti-KRT8 Recombinant Antibody (clone R01-9E6) (CAT#: VS3-XY3413)
It is a recombinant rabbit antibody that recognizes KRT8 (Keratin 8). It is capable of recognizing human and mouse KRT8. It has been used in a variety of applications, such as Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/131/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/131/if_selected.jpg
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach, upper shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/ihc_selected.jpg
(Immunohistochemical staining of human stomach, upper shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/ihc_selected.jpg
Figure 4 Colon
(Endocrine cells
Staining:
High
Intensity: Strong
Enterocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining:
High
Intensity: Strong
Goblet cells
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_7_3.jpg
(Endocrine cells
Staining:
High
Intensity: Strong
Enterocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining:
High
Intensity: Strong
Goblet cells
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_7_3.jpg
Figure 5 Liver
(Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_8_4.jpg
(Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_8_4.jpg
Figure 6 Kidney
(Cells in tubules
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_8_5.jpg
(Cells in tubules
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_8_5.jpg
Figure 7 Testis
(Spermatogonia cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_5_6.jpg
(Spermatogonia cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_5_6.jpg
Figure 8 Prostate
(Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_3_5.jpg
(Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_3_5.jpg
Figure 9 RNA cell line category: Cell line enhanced (A549, BEWO, CACO-2, CAPAN-2, MCF7, SK-BR-3, T-47d)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170421-KRT8
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170421-KRT8
Figure 9 RNA cell line category: Cell line enhanced (A549, BEWO, CACO-2, CAPAN-2, MCF7, SK-BR-3, T-47d)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170421-KRT8
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170421-KRT8
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse KRT8
- Species Reactivity
- Human, Mouse
- Clone
- R01-9E6
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Cryptogenic Cirrhosis; Hidrocystoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
- Gene ID
- 3856
- UniProt ID
- P05787
- Long Name
- Keratin 8
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament, Keratin, Nucleus
- Post Translation Modifications
- Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
- Protein Refseq
- NP_001243222.1; NP_002264.1
- Function
- Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "KRT8"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1511MZ | Recombinant Mouse Anti-Human KRT8 Antibody (clone 2C23) | ICC/IF, WB | Mouse antibody |
| MRO-0452-CN | Recombinant Mouse Anti-KRT8 Monoclonal Antibody (A1-B11) | WB, IF, IHC, FC | Mouse IgG1 |
| ZG-0783F | Mouse Anti-KRT8 Recombinant Antibody (clone C50) | WB, IHC-Fr | Mouse IgG1 |
| VS3-CJ425 | Mouse Anti-KRT8 Recombinant Antibody (clone ABT-CK8) | WB, IHC-P | Mouse IgG1, κ |
| VS3-CJ426 | Mouse Anti-KRT8 Recombinant Antibody (clone PT1903) | WB, IHC-P | Mouse IgG2a, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1993 | Hi-Affi™ Recombinant Rabbit Anti-KRT8 Monoclonal Antibody (DS1993AB) | IHC-P, IHC-Fr | IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0199-WJ-S(P) | Mouse Anti-KRT8 Recombinant Antibody (clone 8G8); scFv Fragment | ELISA, IHC | Mouse scFv |
| FAMAB-0344JF-S(P) | Human Anti-KRT8 Recombinant Antibody (clone COU-1); scFv Fragment | IHC, ELISA, FuncS | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0199-WJ-F(E) | Mouse Anti-KRT8 Recombinant Antibody (clone 8G8); Fab Fragment | ELISA, IHC | Mouse Fab |
| FAMAB-0344JF-F(E) | Human Anti-KRT8 Recombinant Antibody (clone COU-1); Fab Fragment | IHC, ELISA, FuncS | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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