Mouse Anti-LAG3 Recombinant Antibody (clone 8B12E10) (CAT#: ZG-0265U)

Figure 1 Mouse Anti-LAG3 Antibody (ZG-0265U) in WB

Positive WB detected in: 1 lane: Recombinant proteins with LAG3; 2 lane: 293F whole cell lysate transfected with LAG3 All lanes: LAG3 antibody at 1: 2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 76, 88 KDa
Observed band size: 76, 88 KDa
Exposure time: 5min

Figure 2 Mouse Anti-LAG3 Antibody (ZG-0265U) in IHC

IHC image of ZG-0265U diluted at 1:500 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Figure 3 Mouse Anti-LAG3 Antibody (ZG-0265U) in FC

Overlay Peak curve showing A549 cells stained with ZG-0265U (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.