Mouse Anti-MCAM Recombinant Antibody (clone 4D10C7) (CAT#: ZG-0234U)

Figure 1 Mouse Anti-MCAM Antibody (ZG-0234U) in WB

Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate All lanes: CD146 antibody at 1: 500
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 72, 58 kDa
Observed band size: 120 kDa

Figure 2 Mouse Anti-MCAM Antibody (ZG-0234U) in IF

Immunofluorescence staining of Hela cells with ZG-0234U at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).

Figure 3 Mouse Anti-MCAM Antibody (ZG-0234U) in FC

Overlay histogram showing MCF-7 cells stained with ZG-0234U (red line) at 1:400. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.